LOCUS       sequence-99756-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector driving expression of eGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, 
            Sanchez-Adams J, Gersbach CA, Guilak F
  TITLE     Scaffold-mediated lentiviral transduction for functional tissue 
            engineering of cartilage.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 
            10.1073/pnas.1321744111. Epub 2014 Feb 18.
  PUBMED    24550481
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1321744111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E798-806"
COMMENT     Sequence Label: pLVE-eGFP
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(200..219)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     547..568
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
       61 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      121 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      181 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      241 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      301 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      361 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      421 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      481 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      541 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      601 gctgtacaag taatctagct agctagtcga gctcaagctt cgaattcgat atcaagctta
      661 tcgataatca acctctggat tacaaaa
//
