LOCUS       sequence-99757-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector driving expression of eGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, 
            Sanchez-Adams J, Gersbach CA, Guilak F
  TITLE     Scaffold-mediated lentiviral transduction for functional tissue 
            engineering of cartilage.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 
            10.1073/pnas.1321744111. Epub 2014 Feb 18.
  PUBMED    24550481
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1321744111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E798-806"
COMMENT     Sequence Label: pLVE-eGFP
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        157..368
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atctcgacgg tatcgatcac gagactagcc tcgaccatcg atgtcgacga taagctttgc
       61 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      121 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      181 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      241 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      301 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      361 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      421 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      481 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      541 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
      601 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
      661 cgacgc
//
