LOCUS       sequence-9976-ex        1940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  can be used to monitor the ATP:ADP ratio during live-cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Berg J, Hung YP, Yellen G
  TITLE     A genetically encoded fluorescent reporter of ATP:ADP ratio.
  JOURNAL   Nat Methods. 2009 Feb;6(2):161-6. doi: 10.1038/nmeth.1288.
  PUBMED    19122669
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-02"; volume: "6"; issue: 
            "2"; pages: "161-6"
COMMENT     Sequence Label: GW1CMV-Perceval
FEATURES             Location/Qualifiers
     source          1..1940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             381..632
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     569..590
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(693..714)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(954..973)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
       61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc
      121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct
      181 agaccaccat gaaaaaggtt gaatccatca tcaggcccga aaagctggag atcgttaaga
      241 aggctctctc ggacgctgga tatgtgggta tgacagtctc tgaggtcaag ggcacgggcg
      301 tccagggcgg catcgtcgag aggtaccgag gaagggagta ctctgcaggc tacaacagcg
      361 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      421 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcttccag tccaagctga
      541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      601 ggatcactct cggcatggac gagctgtaca agggcggttc cggaggcatg gtgagcaagg
      661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      781 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      841 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1081 acggcaccat agtagatctg atccctaagg taaaaattga gctcgtggtg aaggaggagg
     1141 acgtcgataa cgtgatagac attatttgcg aaaatgcccg cacaggtaac cccggcgacg
     1201 gtaagatatt tgtgatccca gtggagcgag tggtcagggt gcgaaccaaa gaggagggag
     1261 catctggtgg tggatccggt ggaggaggtg cgtctggtat gaaaaaggtg gaagctatca
     1321 tcagacccga aaagctggag atcgttaaaa aggctctttc cgacgccgga tatgtgggta
     1381 tgacagtctc tgaggtcaaa ggccgcggtg caggtggagg ggatctgatc cctaaggtaa
     1441 aaattgagct cgtggtgaag gaggaggacg tcgataacgt gatagacatt atttgcgaaa
     1501 atgcccgcac aggtaacccc ggcgacggta agatatttgt gatcccagtg gagcgagtgg
     1561 tcagggtgcg aaccaaagag gagggagcat ctggtggagg tggcggttcc ggaggtgcat
     1621 ctggtatgaa aaaggtggaa gctatcatca gacccgaaaa gctggagatc gttaaaaagg
     1681 ctctttccga cgccggatat gtgggtatga cagtctctga ggtcaaaggc cgcggtgcag
     1741 gtggagggga tctgatccct aaggtaaaaa ttgagctcgt ggtgaaggag gaggacgtcg
     1801 ataacgtgat agacattatt tgcgaaaatg cccgcacagg taaccccggc gacggtaaga
     1861 tatttgtgat cccagtggag cgagtggtca gggtgcgaac caaagaggag ggaaaggaag
     1921 cattgtgact cgacgaattc
//
