LOCUS       sequence-99767-e        2819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Gateway Entry Clone Containing 4xnrUAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Akitake CM, Macurak M, Halpern ME, Goll MG
  TITLE     Transgenerational analysis of transcriptional silencing in 
            zebrafish.
  JOURNAL   Dev Biol. 2011 Apr 15;352(2):191-201. doi: 
            10.1016/j.ydbio.2011.01.002. Epub 2011 Jan 9.
  PUBMED    21223961
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2011.01.002"; journalName: "Dev Biol"; date: 
            "2011-04-15- 15"; volume: "352"; issue: "2"; pages: "191-201"
COMMENT     Sequence Label: p5E-4xnrUAS (L4-R1)
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    191..314
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(386..405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(387..405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(410..426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(410..426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(528..547)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             539..1348
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(606..625)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1495..2083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1984..2003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2237..2254
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2413..2440)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2532..2618)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2667..2689
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2681..2698
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2682..2698
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(2738..2819,1..14)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtatagaaaa gttgacggtg gcttctaatc cgtgagtcct agcgggtgac agccctccgt
       61 cttcacaggc ggaggagagt cttccgtagg gttcctcgga gtactgtcct ccgacgcgtg
      121 caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct cactttgagc
      181 tcctccacac caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc
      241 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
      301 atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct
      361 agagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
      421 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      481 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      541 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
      601 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
      661 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
      721 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
      781 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
      841 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
      901 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
      961 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1021 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1081 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1141 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1201 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1261 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     1321 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     1381 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     1441 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1501 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1561 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1621 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1681 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1741 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1801 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1861 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     1921 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1981 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2041 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2101 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2161 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2221 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2341 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     2401 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     2461 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     2521 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     2581 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     2641 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     2701 taagctcggg cccgagttaa cgctaccatg gagctccaaa taatgatttt attttgactg
     2761 atagtgacct gttcgttgca acaaattgat aagcaatgct tttttataat gccaacttt
//
