LOCUS       sequence-99775-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-HA
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             501..521
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             540..566
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(623..640)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
       61 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
      121 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
      181 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
      241 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
      301 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
      361 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
      421 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgacccaa
      481 ttgccggatc caaggctagc ccgaaaaaga aacgcaaagt tgggcgcgcc ttaattaact
      541 acccgtacga cgttccggac tacgcttctt gaatcggtag gaattcgcgg ccgtctagac
      601 ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
//
