LOCUS       sequence-99784-e       16863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 
            (aa 2-2414) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 16863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-FLp300
FEATURES             Location/Qualifiers
     source          1..16863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..972
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             979..999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1009..1035,1036..1038,1039..3525,3526..3528,
                     3529..5112)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5137..5157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8305..10155
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      9919..9928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12406..12432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(12457..12474)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12463..12687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12733..13161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12820..12839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13030..13051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13170..13190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13175..13504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13355..13490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13417..13436
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13571..14365
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13625..13644)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14235..14254
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14539..14660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14576..14595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14630..14649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14709..14725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14709..14725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14722..14744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14733..14749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14757..14763,14764..14781,14782..14787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14802..14823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14940..14957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15111..15696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15191..15210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15867..16658,16659..16727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16490..16509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16728..16832)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      961 gacgatgaca agatggcccc caagaagaag aggaaggtgg gccgcggaat ggataagaaa
     1021 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
     1081 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
     1141 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
     1201 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     1261 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     1321 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     1381 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     1441 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     1501 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     1561 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     1621 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     1681 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     1741 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     1801 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     1861 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     1921 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     1981 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     2041 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     2101 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     2161 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     2221 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     2281 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     2341 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     2401 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     2461 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     2521 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     2581 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     2641 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     2701 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     2761 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     2821 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     2881 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     2941 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     3001 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     3061 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     3121 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     3181 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     3241 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     3301 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     3361 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     3421 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     3481 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
     3541 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     3601 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     3661 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     3721 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     3781 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     3841 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     3901 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     3961 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     4021 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     4081 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     4141 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     4201 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     4261 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga
     4321 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     4381 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     4441 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     4501 gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     4561 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     4621 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     4681 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     4741 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     4801 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     4861 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     4921 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     4981 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     5041 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     5101 ctaggaggtg acccaattgc cggatccaag gctagcccga aaaagaaacg caaagttggg
     5161 cgcgccgccg agaatgtggt ggaaccgggg ccgccttcag ccaagcggcc taaactctca
     5221 tctccggccc tctcggcgtc cgccagcgat ggcacagatt ttggctctct atttgacttg
     5281 gagcacgact taccagatga attaatcaac tctacagaat tgggactaac caatggtggt
     5341 gatattaatc agcttcagac aagtcttggc atggtacaag atgcagcttc taaacataaa
     5401 cagctgtcag aattgctgcg atctggtagt tcccctaacc tcaatatggg agttggtggc
     5461 ccaggtcaag tcatggccag ccaggcccaa cagagcagtc ctggattagg tttgataaat
     5521 agcatggtca aaagcccaat gacacaggca ggcttgactt ctcccaacat ggggatgggc
     5581 actagtggac caaatcaggg tcctacgcag tcaacaggta tgatgaacag tccagtaaat
     5641 cagcctgcca tgggaatgaa cacagggatg aatgcgggca tgaatcctgg aatgttggct
     5701 gcaggcaatg gacaagggat aatgcctaat caagtcatga acggttcaat tggagcaggc
     5761 cgagggcgac agaatatgca gtacccaaac ccaggcatgg gaagtgctgg caacttactg
     5821 actgagcctc ttcagcaggg ctctccccag atgggaggac aaacaggatt gagaggcccc
     5881 cagcctctta agatgggaat gatgaacaac cccaatcctt atggttcacc atatactcag
     5941 aatcctggac agcagattgg agccagtggc cttggtctcc agattcagac aaaaactgta
     6001 ctatcaaata acttatctcc atttgctatg gacaaaaagg cagttcctgg tggaggaatg
     6061 cccaacatgg gtcaacagcc agccccgcag gtccagcagc caggtctggt gactccagtt
     6121 gcccaaggga tgggttctgg agcacataca gctgatccag agaagcgcaa gctcatccag
     6181 cagcagcttg ttctcctttt gcatgctcac aagtgccagc gccgggaaca ggccaatggg
     6241 gaagtgaggc agtgcaacct tccccactgt cgcacaatga agaatgtcct aaaccacatg
     6301 acacactgcc agtcaggcaa gtcttgccaa gtggcacact gtgcatcttc tcgacaaatc
     6361 atttcacact ggaagaattg tacaagacat gattgtcctg tgtgtctccc cctcaaaaat
     6421 gctggtgata agagaaatca acaaccaatt ttgactggag cacccgttgg acttggaaat
     6481 cctagctctc taggggtggg tcaacagtct gcccccaacc taagcactgt tagtcagatt
     6541 gatcccagct ccatagaaag agcctatgca gctcttggac taccctatca agtaaatcag
     6601 atgccgacac aaccccaggt gcaagcaaag aaccagcaga atcagcagcc tgggcagtct
     6661 ccccaaggca tgcggcccat gagcaacatg agtgctagtc ctatgggagt aaatggaggt
     6721 gtaggagttc aaacgccgag tcttctttct gactcaatgt tgcattcagc cataaattct
     6781 caaaacccaa tgatgagtga aaatgccagt gtgccctcca tgggtcctat gccaacagca
     6841 gctcaaccat ccactactgg aattcggaaa cagtggcacg aagatattac tcaggatctt
     6901 cgaaatcatc ttgttcacaa actcgtccaa gccatatttc ctacgccgga tcctgctgct
     6961 ttaaaagaca gacggatgga aaacctagtt gcatatgctc ggaaagttga aggggacatg
     7021 tatgaatctg caaacaatcg agcggaatac taccaccttc tagctgagaa aatctataag
     7081 atccagaaag aactagaaga aaaacgaagg accagactac agaagcagaa catgctacca
     7141 aatgctgcag gcatggttcc agtttccatg aatccagggc ctaacatggg acagccgcaa
     7201 ccaggaatga cttctaatgg ccctctacct gacccaagta tgatccgtgg cagtgtgcca
     7261 aaccagatga tgcctcgaat aactccacaa tctggtttga atcaatttgg ccagatgagc
     7321 atggcccagc cccctattgt accccggcaa acccctcctc ttcagcacca tggacagttg
     7381 gctcaacctg gagctctcaa cccgcctatg ggctatgggc ctcgtatgca acagccttcc
     7441 aaccagggcc agttccttcc tcagactcag ttcccatcac agggaatgaa tgtaacaaat
     7501 atccctttgg ctccgtccag cggtcaagct ccagtgtctc aagcacaaat gtctagttct
     7561 tcctgcccgg tgaactctcc tataatgcct ccagggtctc aggggagcca cattcactgt
     7621 ccccagcttc ctcaaccagc tcttcatcag aattcaccct cgcctgtacc tagtcgtacc
     7681 cccacccctc accatactcc cccaagcata ggggctcagc agccaccagc aacaacaatt
     7741 ccagcccctg ttcctacacc tcctgccatg ccacctgggc cacagtccca ggctctacat
     7801 ccccctccaa ggcagacacc tacaccacca acaacacaac ttccccaaca agtgcagcct
     7861 tcacttcctg ctgcaccttc tgctgaccag ccccagcagc agcctcgctc acagcagagc
     7921 acagcagcgt ctgttcctac cccaacagca ccgctgcttc ctccgcagcc tgcaactcca
     7981 ctttcccagc cagctgtaag cattgaagga caggtatcaa atcctccatc tactagtagc
     8041 acagaagtga attctcaggc cattgcggag aagcagcctt cccaggaagt gaagatggag
     8101 gccaaaatgg aagtggatca accagaacca gcagatactc agccggagga tatttcagag
     8161 tctaaagtgg aagactgtaa aatggaatct accgaaacag aagagagaag cactgagtta
     8221 aaaactgaaa taaaagagga ggaagaccag ccaagtactt cagctaccca gtcatctccg
     8281 gctccaggac agtcaaagaa aaagattttc aaaccagaag aactacgaca ggcactgatg
     8341 ccaactttgg aggcacttta ccgtcaggat ccagaatccc ttccctttcg tcaacctgtg
     8401 gaccctcagc ttttaggaat ccctgattac tttgatattg tgaagagccc catggatctt
     8461 tctaccatta agaggaagtt agacactgga cagtatcagg agccctggca gtatgtcgat
     8521 gatatttggc ttatgttcaa taatgcctgg ttatataacc ggaaaacatc acgggtatac
     8581 aaatactgct ccaagctctc tgaggtcttt gaacaagaaa ttgacccagt gatgcaaagc
     8641 cttggatact gttgtggcag aaagttggag ttctctccac agacactgtg ttgctacggc
     8701 aaacagttgt gcacaatacc tcgtgatgcc acttattaca gttaccagaa caggtatcat
     8761 ttctgtgaga agtgtttcaa tgagatccaa ggggagagcg tttctttggg ggatgaccct
     8821 tcccagcctc aaactacaat aaataaagaa caattttcca agagaaaaaa tgacacactg
     8881 gatcctgaac tgtttgttga atgtacagag tgcggaagaa agatgcatca gatctgtgtc
     8941 cttcaccatg agatcatctg gcctgctgga ttcgtctgtg atggctgttt aaagaaaagt
     9001 gcacgaacta ggaaagaaaa taagttttct gctaaaaggt tgccatctac cagacttggc
     9061 acctttctag agaatcgtgt gaatgacttt ctgaggcgac agaatcaccc tgagtcagga
     9121 gaggtcactg ttagagtagt tcatgcttct gacaaaaccg tggaagtaaa accaggcatg
     9181 aaagcaaggt ttgtggacag tggagagatg gcagaatcct ttccataccg aaccaaagcc
     9241 ctctttgcct ttgaagaaat tgatggtgtt gacctgtgct tctttggcat gcatgttcaa
     9301 gagtatggct ctgactgccc tccacccaac cagaggagag tatacatatc ttacctcgat
     9361 agtgttcatt tcttccgtcc taaatgcttg aggactgcag tctatcatga aatcctaatt
     9421 ggatatttag aatatgtcaa gaaattaggt tacacaacag ggcatatttg ggcatgtcca
     9481 ccaagtgagg gagatgatta tatcttccat tgccatcctc ctgaccagaa gatacccaag
     9541 cccaagcgac tgcaggaatg gtacaaaaaa atgcttgaca aggctgtatc agagcgtatt
     9601 gtccatgact acaaggatat ttttaaacaa gctactgaag atagattaac aagtgcaaag
     9661 gaattgcctt atttcgaggg tgatttctgg cccaatgttc tggaagaaag cattaaggaa
     9721 ctggaacagg aggaagaaga gagaaaacga gaggaaaaca ccagcaatga aagcacagat
     9781 gtgaccaagg gagacagcaa aaatgctaaa aagaagaata ataagaaaac cagcaaaaat
     9841 aagagcagcc tgagtagggg caacaagaag aaacccggga tgcccaatgt atctaacgac
     9901 ctctcacaga aactatatgc caccatggag aagcataaag aggtcttctt tgtgatccgc
     9961 ctcattgctg gccctgctgc caactccctg cctcccattg ttgatcctga tcctctcatc
    10021 ccctgcgatc tgatggatgg tcgggatgcg tttctcacgc tggcaaggga caagcacctg
    10081 gagttctctt cactccgaag agcccagtgg tccaccatgt gcatgctggt ggagctgcac
    10141 acgcagagcc aggaccgctt tgtctacacc tgcaatgaat gcaagcacca tgtggagaca
    10201 cgctggcact gtactgtctg tgaggattat gacttgtgta tcacctgcta taacactaaa
    10261 aaccatgacc acaaaatgga gaaactaggc cttggcttag atgatgagag caacaaccag
    10321 caggctgcag ccacccagag cccaggcgat tctcgccgcc tgagtatcca gcgctgcatc
    10381 cagtctctgg tccatgcttg ccagtgtcgg aatgccaatt gctcactgcc atcctgccag
    10441 aagatgaagc gggttgtgca gcataccaag ggttgcaaac ggaaaaccaa tggcgggtgc
    10501 cccatctgca agcagctcat tgccctctgc tgctaccatg ccaagcactg ccaggagaac
    10561 aaatgcccgg tgccgttctg cctaaacatc aagcagaagc tccggcagca acagctgcag
    10621 caccgactac agcaggccca aatgcttcgc aggaggatgg ccagcatgca gcggactggt
    10681 gtggttgggc agcaacaggg cctcccttcc cccactcctg ccactccaac gacaccaact
    10741 ggccaacagc caaccacccc gcagacgccc cagcccactt ctcagcctca gcctacccct
    10801 cccaatagca tgccacccta cttgcccagg actcaagctg ctggccctgt gtcccagggt
    10861 aaggcagcag gccaggtgac ccctccaacc cctcctcaga ctgctcagcc accccttcca
    10921 gggcccccac ctgcagcagt ggaaatggca atgcagattc agagagcagc ggagacgcag
    10981 cgccagatgg cccacgtgca aatttttcaa aggccaatcc aacaccagat gcccccgatg
    11041 actcccatgg cccccatggg tatgaaccca cctcccatga ccagaggtcc cagtgggcat
    11101 ttggagccag ggatgggacc gacagggatg cagcaacagc caccctggag ccaaggagga
    11161 ttgcctcagc cccagcaact acagtctggg atgccaaggc cagccatgat gtcagtggcc
    11221 cagcatggtc aacctttgaa catggctcca caaccaggat tgggccaggt aggtatcagc
    11281 ccactcaaac caggcactgt gtctcaacaa gccttacaaa accttttgcg gactctcagg
    11341 tctcccagct ctcccctgca gcagcaacag gtgcttagta tccttcacgc caacccccag
    11401 ctgttggctg cattcatcaa gcagcgggct gccaagtatg ccaactctaa tccacaaccc
    11461 atccctgggc agcctggcat gccccagggg cagccagggc tacagccacc taccatgcca
    11521 ggtcagcagg gggtccactc caatccagcc atgcagaaca tgaatccaat gcaggcgggc
    11581 gttcagaggg ctggcctgcc ccagcagcaa ccacagcagc aactccagcc acccatggga
    11641 gggatgagcc cccaggctca gcagatgaac atgaaccaca acaccatgcc ttcacaattc
    11701 cgagacatct tgagacgaca gcaaatgatg caacagcagc agcaacaggg agcagggcca
    11761 ggaataggcc ctggaatggc caaccataac cagttccagc aaccccaagg agttggctac
    11821 ccaccacagc agcagcagcg gatgcagcat cacatgcaac agatgcaaca aggaaatatg
    11881 ggacagatag gccagcttcc ccaggccttg ggagcagagg caggtgccag tctacaggcc
    11941 tatcagcagc gactccttca gcaacagatg gggtcccctg ttcagcccaa ccccatgagc
    12001 ccccagcagc atatgctccc aaatcaggcc cagtccccac acctacaagg ccagcagatc
    12061 cctaattctc tctccaatca agtgcgctct ccccagcctg tcccttctcc acggccacag
    12121 tcccagcccc cccactccag tccttcccca aggatgcagc ctcagccttc tccacaccac
    12181 gtttccccac agacaagttc cccacatcct ggactggtag ctgcccaggc caaccccatg
    12241 gaacaagggc attttgccag cccggaccag aattcaatgc tttctcagct tgctagcaat
    12301 ccaggcatgg caaacctcca tggtgcaagc gccacggacc tgggactcag caccgataac
    12361 tcagacttga attcaaacct ctcacagagt acactagaca tacactaccc gtacgacgtt
    12421 ccggactacg cttcttgagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc
    12481 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    12541 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    12601 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    12661 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    12721 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    12781 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    12841 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    12901 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    12961 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    13021 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    13081 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    13141 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
    13201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    13261 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    13321 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    13381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    13441 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    13501 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
    13561 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
    13621 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
    13681 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
    13741 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
    13801 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
    13861 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
    13921 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
    13981 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    14041 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
    14101 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
    14161 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    14221 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    14281 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    14341 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
    14401 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    14461 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    14521 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    14581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    14641 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    14701 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    14761 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    14821 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    14881 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    14941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    15001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    15061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    15121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    15181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    15241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    15301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    15361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    15421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    15481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    15541 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    15601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc
    15661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    15721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    15781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    15841 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    15901 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    15961 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    16021 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    16081 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    16141 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    16201 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    16261 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    16321 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    16381 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    16441 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    16501 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    16561 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    16621 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    16681 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    16741 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    16801 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    16861 gtc
//
