LOCUS       sequence-99787-e        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tet3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Wang Y, Zhang Y
  TITLE     Regulation of TET protein stability by calpains.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):278-84. doi: 
            10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.
  PUBMED    24412366
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.031"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "278-84"
COMMENT     Sequence Label: pcDNA-Flag-Tet3
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttatgga ctacaaagac
      121 gatgacgaca agggatccag ccagtttcag gtgcccctgg ccgtccagcc ggacctgcca
      181 ggcctttatg acttccctca gcgccaggtg atggtaggga gcttcccggg gtctgggctc
      241 tccatggctg ggagtgagtc ccaactccga gggggtggag atggtcgaaa gaaacggaaa
      301 cggtgtggta cttgtgagcc ctgccggcgg ctggaaaact gtggcgcttg cactagctgt
      361 accaaccgcc gcacgcacca gatctgcaaa ctgcgaaaat gtgaggtgct gaagaaaaaa
      421 gtagggcttc tcaaggaggt ggaaataaag gctggtgaag gagccgggcc gtggggacaa
      481 ggagcggctg tcaagacagg ctcagagctc agcccagttg atggacctgt tccaggtcag
      541 atggactcag ggccagtgta ccatggagat tcaaggcagc taagcacctc aggggcgccg
      601 gtcaatggtg ctagagagcc cgccggaccc ggtcttctgg gagctgcggg tccttggcgg
      661 gtagaccaga agcccgactg ggaggctgcc tcaggcccca ctcacgctgc tcgtctggaa
      721 gatgcccacg acctggtggc cttttcggcc gtggccgaag ctgtgtcatc ttacggggcc
      781 cttagtaccc ggctctatga aaccttcaac cgtgagatga gtcgtgaggc tgggagcaac
      841 ggcaggggcc cccggcctga gagctgctct gagggcagtg aagacctgga cacgctgcag
      901 acagccctgg cccttgcaag gcatggcatg aaaccaccca actgcacctg cgatggccca
      961 gagtgccccg acttcctcga gtggctggag ggcaagatca agtctatggc catggaggga
     1021 gggcaggggc ggcctaggct cccaggcgct ctgcctccca gtgaggctgg c
//
