LOCUS       sequence-9979-ex       16583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Ziel JW, Hagedorn EJ, Audhya A, Sherwood DR
  TITLE     UNC-6 (netrin) orients the invasive membrane of the anchor cell in 
            C. elegans.
  JOURNAL   Nat Cell Biol. 2009 Feb . 11(2):183-9.
  PUBMED    19098902
REFERENCE   2  (bases 1 to 16583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2009 Feb . 11(2):183-9."
COMMENT     Sequence Label: pAA173
FEATURES             Location/Qualifiers
     source          1..16583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(14392..14410)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14431..14447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14431..14447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14444..14466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14455..14471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14479..14485,14486..14503,14504..14509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(14662..14679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14833..15421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(15592..16383,16384..16452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16215..16234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16453..16557)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtagcgattg tgtcgattta cgggctcgat tctcggaaaa
     7681 aaaaatcatt tttcaaattt tgacagcgat attcaatttt tcttcgtttt ttcgtatttc
     7741 tcagtcattt ttgtgcttat ttttaatatt ttattcatta ataaattaat ttaattgaaa
     7801 aacgtccaga cgtattttta catttcccgt ctatttccgt aataggcggt taataacgta
     7861 gaaatacgtc tgtatgagtt tactcatcaa aatgacacga atattggacg tttttaaatc
     7921 aaattaattt attaataggt aaaatattaa aaaataaaca caaaaatgac aaaggaatag
     7981 gaaaacacga agagaaattg agtatcgctg tcaaaattcg aaaaacgtgg atttttccaa
     8041 gaatcgagcc cgtaaatcga cacattcgct acagtagtca tttaaggatt attgtagttt
     8101 tcgctacgag atactttgcg cgccaaaaac gtggtgaaat acgcattctc agaattttat
     8161 gttcccgtaa taccacaatg accgaatatc ataataaaaa aattcaaaaa caatttctag
     8221 attttatatg attttctgaa aattgaaaaa atctcagttt tcacctaatt atatttgaac
     8281 tacagccaat tgaactcgtt cgttggagcg cgcttgcatt attttcatta attaatttta
     8341 ttaatcctca ttatttcact gattttcttc atttttgagg ttttttttat cgggaaatga
     8401 aagaaatata caagaaaaat gcaaaatgtt tattaaaaac tgaaattagt atttctcgga
     8461 gttttaggca ttttcagtta ctttttaata aagattttgc atttttcttg tatatttctt
     8521 tcattttccg attaaaaaac cccaaaaatg aagaaaatca gtgaaataat gaaaattaat
     8581 tcaaataact aatgaaaata atgcaagcgc gctccaacca acgagttcaa ttggcggtaa
     8641 ttcaaatata attaggtgaa aactgagatt ttttcaattt tcaaaaaatc atataacatc
     8701 tagacaattt ttttgaattt ttttatcatg atattcggtc attgtgtccc cataggcatg
     8761 ttttaaagca atttccacct ttaaataaaa tcgagaaaaa atggcgtcaa aagacatatg
     8821 taaaagctgt ttctctttgt atattcacac agaatatagc ccgattttgg aggtgaaaaa
     8881 ccggtttttt tgtattcttt acgccacata aagtgatatg gaagagaaaa tcgacatatt
     8941 tatgtacttg gagtacgagt acattggaga aaaagggtga actactggaa aatctgggaa
     9001 atttcagaaa aaaattccga aaaatctttt ttgctgaaaa aaaaatttgt tcgaaatttg
     9061 ggaaatactg gaaaattttc atcaaaatta aagcgatttt taacgttttt gcttgggaga
     9121 tactatttgt aattttaatg tttggtgagg acaacagaaa tgaccttcag cgtacggaaa
     9181 acgattaaaa gacacatttg aatcgaaaat agcgtgaaaa acatgaaaat atcggaaaaa
     9241 tagctttaaa attggatttg aaagcaaaaa atgtatcaaa aatcgagaaa aatgcttgaa
     9301 aaagttgaaa aactcaaaca aaaaatcaaa aaaggttttt ttaaaaatct gtgagtatcg
     9361 caacgaaagc acaacttgag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggaggca
     9421 aaaaaagatt tctcgctgtt ttttcagttt taaaatcact tttcacgttt cttttcgcag
     9481 taattcgttt gcaaaacatc tgaaaaaatt ttattttata agaaattata taaaaatcgc
     9541 tataaattga gtttttgccc ccaaattcag cacggagcac ttctcaatta aatataattt
     9601 tattttttaa cattgaaagc tttcataaaa ttgagctttg tttgtaaata atcagtgaaa
     9661 aaacacagaa atcttcaaaa aataaagaaa aagtttttaa aaatctgtga gtcccgcaac
     9721 gaaaataaaa ctttaggcat gcgcctttaa accaaccgta accaaattcg gcggcaaaaa
     9781 tggatttctc gccgtttttt cagttttaca atcacttttt aggtttcttt ttgcagtatt
     9841 tcgtttccca aacaattaaa aatcaaattt tcttttccag atggctcaaa caaagccgat
     9901 tgccgagcaa atgggatcca tggtctcaaa gggtgaagaa gataacatgg caattattaa
     9961 agagtttatg cgtttcaagg tgcatatgga gggatctgtc aatgggcatg agtttgaaat
    10021 tgaaggtgaa ggagaaggcc gaccatatga gggaacacaa accgcaaaac taaaggtaag
    10081 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtaa ctaaaggcgg
    10141 accattacca ttcgcctggg acatcctctc tccacagttc atgtatggaa gtaaagctta
    10201 tgttaaacat ccggcagata taccagatta tttgaaactt tcattcccgg agggttttaa
    10261 gtgggaacgc gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc aagactcaag
    10321 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gcctccaaga
    10381 tggagaattt atttataaag tcaaacttcg aggaacgaat ttcccctcgg atggacctgt
    10441 tatgcagaag aagactatgg gatgggaagc ttcaagtgaa agaatgtacc ctgaagacgg
    10501 tgctcttaag ggagagatta aacaacgtct taaattgaaa gatggaggac attacgatgc
    10561 tgaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggtgaa
    10621 gacaacttac aaagccaaaa aaccagttca gctgccagga gcgtacaatg ttaatattaa
    10681 actggatatc acctcccaca acgaggatta cactatcgtt gagcaatatg aaagagctga
    10741 agggcggcac tcgacaggtg gcatggatga attgtataag actagtcacg ggctccagga
    10801 tgacccggac cttcaggccc ttctgaaggg cagccagctt ctgaaggtga agtccagctc
    10861 gtggcgtagg gaacgcttct acaagctaca ggaggactgc aagaccatct ggcaggaatc
    10921 tcgaaaggtc atgaggtccc cggagtcgca gctgttctcc atcgaggaca ttcaggaggt
    10981 acggatggga caccgcacag aaggcctgga gaagtttgcc cgagacatcc ccgaggatcg
    11041 atgcttctcc attgtcttca aggaccagcg caacacccta gacctcattg ccccatcacc
    11101 agctgacgct cagcactggg tgcagggcct gcgcaagatc atccaccact ccggctccat
    11161 ggaccagcgg cagaagtaaa ctagtggatc ctaattttgc cgtattttcc atattttgtt
    11221 ttgtatattt atccactcac cccctctctt tgtcctgtga atgaacttgt gccaaaaaag
    11281 ccaaaaataa tttacttttt ttaaagacat attaaaactt gaaaacttgg aacaatacgg
    11341 tgcgaaagcg aaacagagac agaaaaatga acaaagagat ccgtgaaaag tgaacgatga
    11401 tttaggcttc ttgttgggcg gcgtggatga gagtgatgtt gtctcctttg agaagaattc
    11461 tgccgatctt gttgcgtccc ttcgtcttca tgttcacctc ctcggcttcg tcgaacacga
    11521 cattcatgaa ctcgtcgaat ccgatgatgt agccctcgag acggtgggtg acatcctcgt
    11581 acaaccagat ttggacccga gtgcggtttt ggagatatct gaaatttggg aaattgtttt
    11641 ctactgatta gtgaaaaaat attacctgaa gatgaggttc acaggctgaa ccatcacttt
    11701 gttgagcttt ctcgtcgaca tttttgcggg atgatctgta aaaatcaata tattttttga
    11761 aagacacaaa tttttaagat aaatatcaag aaaactcgat tttagcagct atttaagctc
    11821 aaaaactaca atttatggct ttaaaacata atttttcaca ttttacataa atttcaacga
    11881 aaaaaaatgc caaaaaactt caaatttgag cagaaatcgg ctattttctc gacaaaaata
    11941 atttcgaaaa ttcgttcaaa aaagatgagt acgcaagcac cgtgacgcac agttgcggac
    12001 tttgtaaacc gtacccctgc ataaaaaggc ggaattttga atttaataaa atgcagttta
    12061 gaacaaagaa aagcacggag agaattgtga taactaatta tttgtattta attttaaata
    12121 ctacataaaa ctgaagattg gaacgagtta aagctggtat ttgcggttta tagccccaaa
    12181 agttaaggct tgttaggcaa ttatcgataa aaacaacgag aaattcgtga aatcggcaaa
    12241 aatttataga aaacgtttga attaaattaa atttacgctt ttgccgccaa cacctatcga
    12301 gaccctagtt ataggggcgg agcgtcatgg gagcgcgctt gcaactcgat ttcaaaaagt
    12361 ttcgctgttt gtacaacatt ctcagttgtt cttaatcagt taaataagtt tcaaaatttt
    12421 caaaatcata aaacacagtt aatgctaaat taaagtctaa aaaatcaggt gataaggggt
    12481 ttggacgacc aaaaagcgac cttcgctcga aaattgataa actaactaca ctttttccgg
    12541 aaaaatgatg gaaaaatcct gattttcttc gttgaatcaa tttttgtttc atttttaaca
    12601 ctttcacatg aaatatttca gttaaaatgc tcaagctaat tgggtttctt ataattttgg
    12661 gtctcgcaac ggccgacaag tgctcagatt gtttggctca ggggaaacag tgagttggaa
    12721 gcttacaatt ctgagaattc gtattgcgca gcttatttga cgcgcaaaat atgaaatatc
    12781 acgtagcgaa aactacagta attctttaaa taagactact gtagtgccta tgtcgattta
    12841 ctgcctcaac tttcttaatc aagctcgtaa atcgacacca gctctacagt agtcatagtt
    12901 ttcgctacga gatattttgc gcgtcaaata tggtgtgcaa tacgcatttt cagaatttta
    12961 tgttctcgta atataccttt tttcagctgg tgtgttactg taaatgcatg tggatttacg
    13021 ccatgtgtca ctacaattac agtgagtttt tttgaagaaa aaaaaattac ccatttgatc
    13081 gccaattcca tttttcaaaa atcaataaaa aaaaaaaatt tcggcgatca gaaacgttac
    13141 agaaagccct aaactgcccg gaactaccag ataaagccca cgcttacgat gattcattta
    13201 tgaggaataa ggtattgatc acacattctg ctgcctacaa tatggaccca caaaagtgtt
    13261 ttacgtaggt tcaccttcta gataaacctt aaaaatcgat actttcagta gtaaaatgcc
    13321 ttcgatgaaa gtttccaaaa cagttaatgt caattgtgat agatatgggc caagtgagtt
    13381 gttttattag cccacggatt tctggcttcc ctcataaatt gaaaaggaag agattgccga
    13441 actagaccat tttggctcgg cgcgttgcgt gtcacgtcgc ggctcgattg taaaactaaa
    13501 tgtattggtc cgtgtggagt acacgacttt ccctttccgg caggcgattg tcaatggagc
    13561 gcgaaaaatt caatgaggaa ggccagaacc ccgtgtctgt agttttctca aaaagtggca
    13621 aaaactcaat aattgccaat ttttgactgc caataaaaat ttcactaaat ttattaaatt
    13681 tattttaagt tttattgaaa tttttttaaa atttccccac tggcgctaaa aaatccaatt
    13741 ttcagaagca atgtgcttca gcttcacggc catagatact gtccaaaaag tgattgcaat
    13801 gacgtttaga gggacagaag gagcagctca attgtctgaa gaaattttgg atttcttcca
    13861 gtaagttcgg gaaatggcaa tttaaagttt aattggattt ttgtattttt tcttctcacg
    13921 actacagttt tgcacatttt tatgttataa aaaaccaaaa aaaaattttt ttgccttttt
    13981 tctcagctta ccacaaaaca atttcgacca aaaataaatt gaaaaaatta aaaaattttt
    14041 tgcacggttt tcggactcaa aaaaagctga atgttcctgg aactatttgt aaaattcaaa
    14101 attcaaaatt ttcacgaatt ctctaaaagg ttccagcaaa ttccacaact ttcttgaatt
    14161 tttcaaaaaa gttcttgagc attcaacaat tatttttgga attttccaaa ggttacagga
    14221 cgttccaatt tttcaaaaaa atttctcgca aaaaatgttt tctcagaaaa tttgaatttc
    14281 cctccacata aaagattgtt ctcaaaaaat ttgcaaacac acattttcag cggaaaaaag
    14341 gcgttcttcg actctggaag tgtattcgaa tatggtaccc agcttttgtt ccctttagtg
    14401 agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    14461 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
    14521 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    14581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    14641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    14701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    14761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    14821 gttgctggcg tttttccata ggctcggccc ccctgacgag catcacaaaa atcgacgctc
    14881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgttcc cccctggaag
    14941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    15001 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
    15061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    15121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    15181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    15241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    15301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    15361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    15421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    15481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    15541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    15601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    15661 actgcccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    15721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    15781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    15841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    15901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    15961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgaaaaaaag cggttagctc
    16021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatgcttat
    16081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    16141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    16201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    16261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    16321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    16381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    16441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    16501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    16561 atttccccga aaagtgccac ctg
//
