LOCUS       sequence-99799-e        2004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutations 
            H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA-dCas9-p300 Core
            (H1415A/E1423A/Y1424A/L1...
FEATURES             Location/Qualifiers
     source          1..2004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             68..1918
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYAEILIGYLAAVKKSGATTGAIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      1682..1691
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1919..1945
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1970..1987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gctaggaggt gacccaattg ccggatccaa ggctagcccg aaaaagaaac gcaaagttgg
       61 gcgcgccatt ttcaaaccag aagaactacg acaggcactg atgccaactt tggaggcact
      121 ttaccgtcag gatccagaat cccttccctt tcgtcaacct gtggaccctc agcttttagg
      181 aatccctgat tactttgata ttgtgaagag ccccatggat ctttctacca ttaagaggaa
      241 gttagacact ggacagtatc aggagccctg gcagtatgtc gatgatattt ggcttatgtt
      301 caataatgcc tggttatata accggaaaac atcacgggta tacaaatact gctccaagct
      361 ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa agccttggat actgttgtgg
      421 cagaaagttg gagttctctc cacagacact gtgttgctac ggcaaacagt tgtgcacaat
      481 acctcgtgat gccacttatt acagttacca gaacaggtat catttctgtg agaagtgttt
      541 caatgagatc caaggggaga gcgtttcttt gggggatgac ccttcccagc ctcaaactac
      601 aataaataaa gaacaatttt ccaagagaaa aaatgacaca ctggatcctg aactgtttgt
      661 tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt gtccttcacc atgagatcat
      721 ctggcctgct ggattcgtct gtgatggctg tttaaagaaa agtgcacgaa ctaggaaaga
      781 aaataagttt tctgctaaaa ggttgccatc taccagactt ggcacctttc tagagaatcg
      841 tgtgaatgac tttctgaggc gacagaatca ccctgagtca ggagaggtca ctgttagagt
      901 agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc atgaaagcaa ggtttgtgga
      961 cagtggagag atggcagaat cctttccata ccgaaccaaa gccctctttg cctttgaaga
     1021 aattgatggt gttgacctgt gcttctttgg catgcatgtt caagagtatg gctctgactg
     1081 ccctccaccc aaccagagga gagtatacat atcttacctc gatagtgttc atttcttccg
     1141 tcctaaatgc ttgaggactg cagtctatgc cgaaatccta attggatatt tagcggccgt
     1201 caagaaatcc ggagccacaa caggggccat ttgggcatgt ccaccaagtg agggagatga
     1261 ttatatcttc cattgccatc ctcctgacca gaagataccc aagcccaagc gactgcagga
     1321 atggtacaaa aaaatgcttg acaaggctgt atcagagcgt attgtccatg actacaagga
     1381 tatttttaaa caagctactg aagatagatt aacaagtgca aaggaattgc cttatttcga
     1441 gggtgatttc tggcccaatg ttctggaaga aagcattaag gaactggaac aggaggaaga
     1501 agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca gatgtgacca agggagacag
     1561 caaaaatgct aaaaagaaga ataataagaa aaccagcaaa aataagagca gcctgagtag
     1621 gggcaacaag aagaaacccg ggatgcccaa tgtatctaac gacctctcac agaaactata
     1681 tgccaccatg gagaagcata aagaggtctt ctttgtgatc cgcctcattg ctggccctgc
     1741 tgccaactcc ctgcctccca ttgttgatcc tgatcctctc atcccctgcg atctgatgga
     1801 tggtcgggat gcgtttctca cgctggcaag ggacaagcac ctggagttct cttcactccg
     1861 aagagcccag tggtccacca tgtgcatgct ggtggagctg cacacgcaga gccaggacta
     1921 cccgtacgac gttccggact acgcttcttg agtttaaacc gctgatcagc ctcgactgtg
     1981 ccttctagtt gccagccatc tgtt
//
