LOCUS       sequence-99801-e        2604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes N. meningiditis dCas9 with c-terminal fusion of human p300 
            HAT core (aa 1048-1664) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3199"; 
            journalName: "Nat Biotechnol"; date: "2015-05"; volume: "33"; issue:
            "5"; pages: "510-7"
COMMENT     Sequence Label: pcDNA3.3-Nm-dCas9-p300 Core
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      131..140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             557..577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             596..2446
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      2210..2219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2447..2473
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2516..2535)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ggccttcgcc gagcccttct acaagtacga caaggccggc aaccgcaccc agcaggtgaa
       61 ggccgtgcgc gtggagcagg tgcagaagac cggcgtgtgg gtgcgcaacc acaacggcat
      121 cgccgacaac gccaccatgg tgcgcgtgga cgtgttcgag aagggcgaca agtactacct
      181 ggtgcccatc tacagctggc aggtggccaa gggcatcctg cccgaccgcg ccgtggtgca
      241 gggcaaggac gaggaggact ggcagctgat cgacgacagc ttcaacttca agttcagcct
      301 gcaccccaac gacctggtgg aggtgatcac caagaaggcc cgcatgttcg gctacttcgc
      361 cagctgccac cgcggcaccg gcaacatcaa catccgcatc cacgacctgg accacaagat
      421 cggcaagaac ggcatcctgg agggcatcgg cgtgaagacc gccctgagct tccagaagta
      481 ccagatcgac gagctgggca aggagatccg cccctgccgc ctgaagaagc gccctcctgt
      541 gcgcagcagg gctgacccca agaagaagag gaaggtggag gccagcgggc gcgccatttt
      601 caaaccagaa gaactacgac aggcactgat gccaactttg gaggcacttt accgtcagga
      661 tccagaatcc cttccctttc gtcaacctgt ggaccctcag cttttaggaa tccctgatta
      721 ctttgatatt gtgaagagcc ccatggatct ttctaccatt aagaggaagt tagacactgg
      781 acagtatcag gagccctggc agtatgtcga tgatatttgg cttatgttca ataatgcctg
      841 gttatataac cggaaaacat cacgggtata caaatactgc tccaagctct ctgaggtctt
      901 tgaacaagaa attgacccag tgatgcaaag ccttggatac tgttgtggca gaaagttgga
      961 gttctctcca cagacactgt gttgctacgg caaacagttg tgcacaatac ctcgtgatgc
     1021 cacttattac agttaccaga acaggtatca tttctgtgag aagtgtttca atgagatcca
     1081 aggggagagc gtttctttgg gggatgaccc ttcccagcct caaactacaa taaataaaga
     1141 acaattttcc aagagaaaaa atgacacact ggatcctgaa ctgtttgttg aatgtacaga
     1201 gtgcggaaga aagatgcatc agatctgtgt ccttcaccat gagatcatct ggcctgctgg
     1261 attcgtctgt gatggctgtt taaagaaaag tgcacgaact aggaaagaaa ataagttttc
     1321 tgctaaaagg ttgccatcta ccagacttgg cacctttcta gagaatcgtg tgaatgactt
     1381 tctgaggcga cagaatcacc ctgagtcagg agaggtcact gttagagtag ttcatgcttc
     1441 tgacaaaacc gtggaagtaa aaccaggcat gaaagcaagg tttgtggaca gtggagagat
     1501 ggcagaatcc tttccatacc gaaccaaagc cctctttgcc tttgaagaaa ttgatggtgt
     1561 tgacctgtgc ttctttggca tgcatgttca agagtatggc tctgactgcc ctccacccaa
     1621 ccagaggaga gtatacatat cttacctcga tagtgttcat ttcttccgtc ctaaatgctt
     1681 gaggactgca gtctatcatg aaatcctaat tggatattta gaatatgtca agaaattagg
     1741 ttacacaaca gggcatattt gggcatgtcc accaagtgag ggagatgatt atatcttcca
     1801 ttgccatcct cctgaccaga agatacccaa gcccaagcga ctgcaggaat ggtacaaaaa
     1861 aatgcttgac aaggctgtat cagagcgtat tgtccatgac tacaaggata tttttaaaca
     1921 agctactgaa gatagattaa caagtgcaaa ggaattgcct tatttcgagg gtgatttctg
     1981 gcccaatgtt ctggaagaaa gcattaagga actggaacag gaggaagaag agagaaaacg
     2041 agaggaaaac accagcaatg aaagcacaga tgtgaccaag ggagacagca aaaatgctaa
     2101 aaagaagaat aataagaaaa ccagcaaaaa taagagcagc ctgagtaggg gcaacaagaa
     2161 gaaacccggg atgcccaatg tatctaacga cctctcacag aaactatatg ccaccatgga
     2221 gaagcataaa gaggtcttct ttgtgatccg cctcattgct ggccctgctg ccaactccct
     2281 gcctcccatt gttgatcctg atcctctcat cccctgcgat ctgatggatg gtcgggatgc
     2341 gtttctcacg ctggcaaggg acaagcacct ggagttctct tcactccgaa gagcccagtg
     2401 gtccaccatg tgcatgctgg tggagctgca cacgcagagc caggactacc cgtacgacgt
     2461 tccggactac gcttcttgaa ccggttagta atgagtttaa acgggggagg ctaactgaaa
     2521 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     2581 aaacgcacgg gtgttgggtc gttt
//
