LOCUS       sequence-99804-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human DNMT3B1 with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
  TITLE     Physical and functional interactions between the human DNMT3L 
            protein and members of the de novo methyltransferase family.
  JOURNAL   J Cell Biochem. 2005 Aug 1;95(5):902-17.
  PUBMED    15861382
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2005-08-1"; volume: "95(5)"; pages: "902-17"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT3B1
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgccacca tggagcagaa gctgatctca gaggaggacc
      121 tggaattcat gaagggagac accaggcatc tcaatggaga ggaggacgcc ggcgggaggg
      181 aagactcgat cctcgtcaac ggggcctgca gcgaccagtc ctccgactcg cccccaatcc
      241 tggaggctat ccgcaccccg gagatcagag gccgaagatc aagctcgcga ctctccaaga
      301 gggaggtgtc cagtctgcta agctacacac aggacttgac aggcgatggc gacggggaag
      361 atggggatgg ctctgacacc ccagtcatgc caaagctctt ccgggaaacc aggactcgtt
      421 cagaaagccc agctgtccga actcgaaata acaacagtgt ctccagccgg gagaggcaca
      481 ggccttcccc acgttccacc cgaggccggc agggccgcaa ccatgtggac gagtcccccg
      541 tggagttccc ggctaccagg tccctgagac ggcgggcaac agcatcggca ggaacgccat
      601 ggccgtcccc tcccagctct taccttacca tcgacctcac agacgacaca gaggacacac
      661 atgggacgcc ccagagcagc agtaccccct acgcccgcct agcccaggac agccagcagg
      721 ggggcatgga gtccccgcag gtggaggcag acagtggaga tggagacagt tcagagtatc
      781 aggat
//
