LOCUS       sequence-99820-e       11140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus 
            pluvialis fused to a Trc promoter. Produces canthaxanthin in E. 
            coli. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosynth 
            Res."; date: "2007-05"; volume: "92(2)"; pages: "245-59. Epub 2007 
            Jul 17"
COMMENT     Sequence Label: pAC-CANTHipi
FEATURES             Location/Qualifiers
     source          1..11140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2042..2047,2048..2064,2065..2071)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2079..2095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2084..2106
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     misc_feature    2112..2181
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region 
                     (Berg et al., 1989)"
     CDS             2232..2258
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             2274..2291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2295..2327
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2331..2339,2340..2354)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(10533..10552)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagctat ctcggtagtg ggatacgacg ataccgaaga
     1561 cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc tgctggggca
     1621 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1681 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1801 cgggcagtga gcgcaacgca attaatgtga gttagcgcga attgatctgg tttgacagct
     1861 tatcatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
     1921 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
     1981 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
     2041 gttgacaatt aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     2101 cacaggaaac agcgccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag
     2161 acaatctgtg tgggcactcg accggaatta tcgattaact ttattattaa aaattaaaga
     2221 ggtatatatt aatgtatcga ttaaataagg aggaataaac catggggggt tctcatcatc
     2281 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
     2341 acgatgacga taaggatcga tggggatccg agctcaagcg tttgtgcgcc tcgacgtggc
     2401 cagtctgcac tgccttgaac ccgcgagtct cccgccgcac tgactgccat agcacagcta
     2461 gacgaatgca gctagcagcg acagtaatgt tggagcagct taccggaagc gctgaggcac
     2521 tcaaggagaa ggagaaggag gttgcaggca gctctgacgt gttgcgtaca tgggcgaccc
     2581 agtactcgct tccgtcagaa gagtcagacg cggcccgccc gggactgaag aatgcctaca
     2641 agccaccacc ttccgacaca aagggcatca caatggcgct agctgtcatc ggctcctggg
     2701 ccgcagtgtt cctccacgcc atttttcaaa tcaagcttcc gacctccttg gaccagctgc
     2761 actggctgcc cgtgtcagat gccacagctc agctggttag cggcagcagc agcctgctgc
     2821 acatcgtcgt agtattcttt gtcctggagt tcctgtacac aggccttttt atcaccacgc
     2881 atgatgctat gcatggcacc atcgccatga gaaacaggca gcttaatgac ttcttgggca
     2941 gagtatgcat ctccttgtac gcctggtttg attacaacat gctgcaccgc aagcattggg
     3001 agcaccacaa ccacactggc gaggtgggca aggaccctga cttccacagg ggaaaccctg
     3061 gcattgtgcc ctggtttgcc agcttcatgt ccagctacat gtcgatgtgg cagtttgcgc
     3121 gcctcgcatg gtggacggtg gtcatgcagc tgctgggtgc gccaatggcg aacctgctgg
     3181 tgttcatggc ggccgcgccc atcctgtccg ccttccgctt gttctacttt ggcacgtaca
     3241 tgccccacaa gcctgagcct ggcgccgcgt caggctcttc accagccgtc atgaactggt
     3301 ggaagtcgcg cactagccag gcgtccgacc tggtcagctt tctgacctgc taccacttcg
     3361 acctgcactg ggagcaccac cgctggccct tcgccccctg gtgggagctg cccaactgcc
     3421 gccgcctgtc tggccgaggt ctggttcctg cctagctgga cacacagctt taatgcggta
     3481 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
     3541 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
     3601 tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg
     3661 tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
     3721 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
     3781 acgcgatcat ggcgaccaca cccgtcctgt ggatctaaag gcacagcgtc tcatgcttcg
     3841 cacaatgtaa aactgcttca gaacctggcg agagctatcc gcgcggtcta cggttaactg
     3901 atactaaaag acaattcagc gggtaacctt gcaatggtga gtggcagtaa agcgggcgtt
     3961 tcgcctcatc gcgaaataga agtaatgaga caatccattg acgatcacct ggctggcctg
     4021 ttacctgaaa ccgacagcca ggatatcgtc agccttgcga tgcgtgaagg cgtcatggca
     4081 cccggtaaac ggatccgtcc gctgctgatg ctgctggccg cccgcgacct ccgctaccag
     4141 ggcagtatgc ctacgctgct cgatctcgcc tgcgccgttg aactgaccca taccgcgtcg
     4201 ctgatgctcg acgacatgcc ctgcatggac aacgccgagc tgcgccgcgg tcagcccact
     4261 acccacaaaa aatttggtga gagcgtggcg atccttgcct ccgttgggct gctctctaaa
     4321 gcctttggtc tgatcgccgc caccggcgat ctgccggggg agaggcgtgc ccaggcggtc
     4381 aacgagctct ctaccgccgt gggcgtgcag ggcctggtac tggggcagtt tcgcgatctt
     4441 aacgatgccg ccctcgaccg tacccctgac gctatcctca gcaccaacca cctcaagacc
     4501 ggcattctgt tcagcgcgat gctgcagatc gtcgccattg cttccgcctc gtcgccgagc
     4561 acgcgagaga cgctgcacgc cttcgccctc gacttcggcc aggcgtttca actgctggac
     4621 gatctgcgtg acgatcaccc ggaaaccggt aaagatcgca ataaggacgc gggaaaatcg
     4681 acgctggtca accggctggg cgcagacgcg gcccggcaaa agctgcgcga gcatattgat
     4741 tccgccgaca aacacctcac ttttgcctgt ccgcagggcg gcgccatccg acagtttatg
     4801 catctgtggt ttggccatca ccttgccgac tggtcaccgg tcatgaaaat cgcctgatac
     4861 cgcccttttg ggttcaagca gtacataacg atggaaccac attacaggag tagtgatgaa
     4921 tgaaggacga gcgccttgtt cagcgtaaga acgatcatct ggatatcgtt ctcgaccccc
     4981 gtcgcgccgt aactcaggct agcgcaggtt ttgagcgctg gcgctttacc cactgcgccc
     5041 tgccagagct gaattttagc gacatcacgc tggaaaccac cttcctgaat cggcagctac
     5101 aggctccgct gctgatcagc tccatgaccg gcggcgttga gcgctcgcgc catatcaacc
     5161 gccacctcgc cgaggcggcg caggtgctaa aaattgcgat gggggtgggc tcccagcgcg
     5221 tcgccattga gagcgacgcg ggcttagggc tggataaaac cctgcggcag ctggctccgg
     5281 acgtgccgct gctggcgaac ctcggcgcgg cgcagctgac cggcagaaaa ggtattgatt
     5341 acgcccgacg ggccgtggag atgatcgagg cggatgcgct gattgtgcac ctaaacccgc
     5401 tgcaggaggc gctacagccc ggcggcgatc gcgactggcg cggacggctg gcggctattg
     5461 aaactctggt ccgcgagctg cccgttccgc tggtggtgaa agaggtggga gccggtatct
     5521 cccgaaccgt ggccgggcag ctgatcgatg ccggcgttac cgtgattgac gtcgcgggcg
     5581 cgggcggcac cagctgggcc gccgttgaag gcgagcgggc ggccaccgag cagcagcgca
     5641 gcgtggccaa cgtctttgcc gactggggga tccccaccgc tgaggcgctg gttgacattg
     5701 ccgaggcctg gccgcagatg ccccttattg cctcgggcgg gattaaaaac ggcgtcgacg
     5761 cggcgaaagc gctgcggctc ggcgcgtgca tggtagggca ggccgccgcc gtgctcggca
     5821 gcgcaggcgt ctccacggag aaggtgatcg atcacttcaa cgtgattatt gagcagctgc
     5881 gggtggcctg cttctgcacc ggcagccgca gcctgagcga tctaaagcag gctgatatcc
     5941 gctatgtgcg ggatacgcca tgagccattt tgccattgtg gcaccgccgc tctacagtca
     6001 tgcggtggcg ctgcatgccc tggcgctgga gatggcccaa cgcggccact cgaccagccg
     6061 cgctacgttg ccgaggctaa tctggtgatc acccacggcg gtctcaatac cgtactggat
     6121 gcgctggctg ccgcgacgcc ggtgctggcg gtgccactct ctttcgacca gcccgccgtg
     6181 gctgcccggc tggtctataa cgggctgggt cgccgggtat cgcgctttgc cagacagcag
     6241 acgctggcgg atgagattgc ccaactgctg ggggatgaga cgctgcatca gcgtctggcg
     6301 acggcccgcc agcagcttaa cgacgccggg ggcacgcccc gtgcggcgac cctgattgaa
     6361 caggccatag cagggagtga gagcgtatcg tgagggatct gattttagtc ggcggcggcc
     6421 tggccaacgg gctgatcgcc tggcgtctgc gccagcgcta cccgcagctt aacctgctgc
     6481 tgatcgaggc cggggagcag cccggcggga accatacctg gtcattccat gaagacgatc
     6541 tgactcccgg gcagcacgcc tggctggccc cgctggtggc ccacgcctgg ccgggctatg
     6601 aggtgcagtt tcccgatctt cgccgtcgcc tcgcgcgcgg ctactactcc attacctcag
     6661 agcgctttgc cgaggccctg catcaggcgc tgggggagaa catctggcta aactgttcgg
     6721 tgagcgaggt gttacccaat agcgtgcgcc ttgccaacgg tgaggcgctg cttgccggag
     6781 cggtgattga cggacgcggc gtgaccgcca gttcggcgat gcaaaccggc tatcagctct
     6841 ttcttggtca gcagtggcgg ctgacacagc cccacggcct gaccgtaccg atcctgatgg
     6901 atgccacggt ggcgcagcag cagggctatc gctttgtcta cacgctgccg ctctccgccg
     6961 acacgctgct gatcgaggat acgcgctacg ccaatgtccc gcagcgtgat gataatgccc
     7021 tacgccagac ggttaccgac tatgctcaca gcaaagggtg gcagctggcc cagcttgaac
     7081 gcgaggagac cggctgtctg ccgattaccc tggcgggtga catccaggct ctgtgggccg
     7141 atgcgccggg cgtgccgcgc tcgggaatgc gggctgggct atttcaccct accactggct
     7201 attcgctgcc gctggcggtg gcccttgccg acgcgattgc cgacagcccg cggctgggca
     7261 gcgttccgct ctatcagctc acccggcagt ttgccgaacg ccactggcgc aggcagggat
     7321 tcttccgcct gctgaaccgg atgcttttcc tggccgggcg cgaggagaac cgctggcggg
     7381 tgatgcagcg cttttatggg ctgccggagc ccaccgtaga gcgcttttac gccggtcggc
     7441 tctctctctt tgataaggcc cgcattttga cgggcaagcc accggttccg ctgggcgaag
     7501 cctggcgggc ggcgctgaac cattttcctg acagacgaga taaaggatga aaaaaaccgt
     7561 tgtgattggc gcaggctttg gtggcctggc gctggcgatt cgcctgcagg cggcagggat
     7621 cccaaccgta ctgctggagc agcgggacaa gcccggcggt cgggcctacg tctggcatga
     7681 ccagggcttt acctttgacg ccgggccgac ggtgatcacc gatcctaccg cgcttgaggc
     7741 gctgttcacc ctggccggca ggcgcatgga ggattacgtc aggctgctgc cggtaaaacc
     7801 cttctaccga ctctgctggg agtccgggaa gaccctcgac tatgctaacg acagcgccga
     7861 gcttgaggcg cagattaccc agttcaaccc ccgcgacgtc gagggctacc ggcgctttct
     7921 ggcttactcc caggcggtat tccaggaggg atatttgcgc ctcggcagcg tgccgttcct
     7981 ctcttttcgc gacatgctgc gcgccgggcc gcagctgctt aagctccagg cgtggcagag
     8041 cgtctaccag tcggtttcgc gctttattga ggatgagcat ctgcggcagg ccttctcgtt
     8101 ccactccctg ctggtaggcg gcaacccctt caccacctcg tccatctaca ccctgatcca
     8161 cgcccttgag cgggagtggg gggtctggtt ccctgagggc ggcaccgggg cgctggtgaa
     8221 cggcatggtg aagctgttta ccgatctggg cggggagatc gaactcaacg cccgggtcga
     8281 agagctggtg gtggccgata accgcgtaag ccaggtccgg ctggcggatg gtcggatctt
     8341 tgacaccgac gccgtagcct cgaacgctga cgtggtgaac acctataaaa agctgctcgg
     8401 ccaccatccg gtggggcaga agcgggcggc agcgctggag cgcaagagca tgagcaactc
     8461 gctgtttgtg ctctacttcg gcctgaacca gcctcattcc cagctggcgc accataccat
     8521 ctgttttggt ccccgctacc gggagctgat cgacgagatc tttaccggca gcgcgctggc
     8581 ggatgacttc tcgctctacc tgcactcgcc ctgcgtgacc gatccctcgc tcgcgcctcc
     8641 cggctgcgcc agcttctacg tgctggcccc ggtgccgcat cttggcaacg cgccgctgga
     8701 ctgggcgcag gaggggccga agctgcgcga ccgcatcttt gactaccttg aagagcgcta
     8761 tatgcccggc ctgcgtagcc agctggtgac ccagcggatc tttaccccgg cagacttcca
     8821 cgacacgctg gatgcgcatc tgggatcggc cttctccatc gagccgctgc tgacccaaag
     8881 cgcctggttc cgcccgcaca accgcgacag cgacattgcc aacctctacc tggtgggcgc
     8941 aggtactcac cctggggcgg gcattcctgg cgtagtggcc tcggcgaaag ccaccgccag
     9001 cctgatgatt gaggatctgc aatgagccaa ccgccgctgc ttgaccacgc cacgcagacc
     9061 atggccaacg gctcgaaaag ttttgccacc gctgcgaagc tgttcgaccc ggccacccgc
     9121 cgtagcgtgc tgatgctcta cacctggtgc cgccactgcg atgacgtcat tgacgaccag
     9181 acccacggct tcgccagcga ggccgcggcg gaggaggagg ccacccagcg cctggcccgg
     9241 ctgcgcacgc tgaccctggc ggcgtttgaa ggggccgaga tgcaggatcc ggccttcgct
     9301 gcctttcagg aggtggcgct gacccacggt attacgcccc gcatggcgct cgatcacctc
     9361 gacggctttg cgatggacgt ggctcagacc cgctatgtca cctttgagga tacgctgcgc
     9421 tactgctatc acgtggcggg cgtggtgggt ctgatgatgg ccagggtgat gggcgtgcgg
     9481 gatgagcggg tgctggatcg cgcctgcgat ctggggctgg ccttccagct gacgaatatc
     9541 gcccgggata ttattgacga tgcggctatt gaccgctgct atctgcccgc cgagtggctg
     9601 caggatgccg ggctgacccc ggagaactat gccgcgcggg agaatcgggc cgcgctggcg
     9661 cgggtggcgg agcggcttat tgatgccgca gagccgtact acatctcctc ccaggccggg
     9721 ctacacgatc tgccgccgcg ctgcgcctgg gcgatcgcca ccgcccgcag cgtctaccgg
     9781 gagatcggta ttaaggtaaa agcggcggga ggcagcgcct gggatcgccg ccagcacacc
     9841 agcaaaggtg aaaaaattgc catgctgatg gcggcaccgg ggcaggttat tcgggcgaag
     9901 acgacgaggg tgacgccgcg tccggccggt ctttggcagc gtcccgttta ggcgggcggc
     9961 catgacgttc acgcaggatc gcctgtaggt cggcaggctt gcgggcgtaa ataaaaccga
    10021 aggagacgca gccctcccgg ccgcgcaccg cgtggtgcag gcggtgggcg acgtagagcc
    10081 gcttcaggta gccccggcgc gggatcccgc ctcgccgtgc tgtccggtct caacctgatc
    10141 cgccagaatc gagccaacgg gatcggccag cacgaattcg gtatgcggag agtgtttggc
    10201 aaaccatgcc tgcaagccac ccagcgtgcc gccggagcca acgcctacta ccacggcatc
    10261 aaccctgcct gccagctggt cgaacagctc cggtgcggtg gtggtggcgt gcgccagcgg
    10321 gttggccggg ttagagaact gatcaatata gtaagcaccc ggcgtctcct ctgccaggcg
    10381 gcgggcgtag tcctgatagt actccgggtg gccctttgtc acgtcggagc gggtcaggcg
    10441 cacatcaaca cccagcgcac gcaggtggta gatcctctac gccggacgca tcgtggccgg
    10501 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
    10561 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
    10621 ccccgtggcc gggggactgt tgggcgccat ctccttgggg tcgaatttgc tttcgaattt
    10681 ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc
    10741 cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca
    10801 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
    10861 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
    10921 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
    10981 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
    11041 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
    11101 tatcccatat caccagctca ccgtctttca ttgccatacg
//
