LOCUS       sequence-99829-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector, 25 repeats of 19 bp BoxB sequence from 
            bacteriophage-lambda, for RNA labeling. BoxB-RNA is bound by 22 
            amino acid RNA binding domain of lambda-anti-terminator protein N, 
            e.g. tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Martin RM, Rino J, Carvalho C, Kirchhausen T, Carmo-Fonseca M
  TITLE     Live-cell visualization of pre-mRNA splicing with single-molecule 
            sensitivity.
  JOURNAL   Cell Rep. 2013 Sep 26;4(6):1144-55. doi: 
            10.1016/j.celrep.2013.08.013. Epub 2013 Sep 12.
  PUBMED    24035393
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.08.013"; journalName: "Cell Rep"; date: 
            "2013-09-26- 26"; volume: "4"; issue: "6"; pages: "1144-55"
COMMENT     Sequence Label: pCMV5-25BoxB
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    194..212
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    218..236
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    277..295
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    301..319
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    345..363
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    401..419
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    425..443
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    484..502
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    508..526
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    552..570
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    608..626
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    632..650
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    691..709
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
       61 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      121 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg ggatccagat
      181 aatatcctcg atagggccct tcttcagggc cctatatggg cccttcttca gggcccagtt
      241 tagtagttgg acttagtcga gataatatcc tcgatagggc ccttcttcag ggccctatat
      301 gggcccttct tcagggccca gtttagtagt tggacttagt cgaggggccc ttcttcaggg
      361 cccaggaatc cccagttaga tccagataat atcctcgata gggcccttct tcagggccct
      421 atatgggccc ttcttcaggg cccagtttag tagttggact tagtcgagat aatatcctcg
      481 atagggccct tcttcagggc cctatatggg cccttcttca gggcccagtt tagtagttgg
      541 acttagtcga ggggcccttc ttcagggccc aggaatcccc agttagatcc agataatatc
      601 ctcgataggg cccttcttca gggccctata tgggcccttc ttcagggccc agtttagtag
      661 ttggacttag tcgagataat atcctcgata gggcccttct tcagggccct atatgggccc
      721 ttc
//
