LOCUS       sequence-99836-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fusion protein of the SunTag10xGCN4 v4 peptide array to a 
            mitochondrial targeting domain and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-mito-mCherry-10xGCN4_v4
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(544..562)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     812..831
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc cgccaccatg agtctgactt ccagttccag cgtacgagtt gaatggatcg
      241 cagcagttac cattgctgct gggacagctg caattggtta tctagcttac aaaagatttt
      301 atgttaaaga tcatcgaaat aaagctatga taaaccttca catccagaaa gacaacccca
      361 agatagtaca tgctggaagc ttaccggtcg ccgaattcgg tgtgagcaag ggcgaggagg
      421 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      481 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      541 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      601 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      661 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      721 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      781 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      841 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctg
//
