LOCUS       sequence-99837-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fusion protein of the SunTag10xGCN4 v4 peptide array to a 
            mitochondrial targeting domain and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-mito-mCherry-10xGCN4_v4
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             325..381
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             397..453
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             469..525
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             541..597
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             613..669
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             685..741
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             757..813
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             829..885
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
       61 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      121 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      181 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      241 gagctgtaca agggtggtgg tggcgcggcc gcagatcgat cgatcacgcg tgccaccatg
      301 actagttttg tacaaagcgg tccggaagaa cttttgagca agaattatca tcttgagaac
      361 gaagtggctc gtcttaagaa aggttctggc agtggagaag aactgctttc aaagaattac
      421 cacctggaaa atgaggtagc tagactgaaa aaggggagcg gaagtgggga ggagttgctg
      481 agcaaaaatt atcatttgga gaacgaagta gcacgactaa agaaagggtc cggatcgggt
      541 gaggagttac tctcgaaaaa ttatcatctc gaaaacgaag tggctcggct aaaaaagggc
      601 agtggttctg gagaagagct attatctaaa aactaccacc tcgaaaatga ggtggcacgc
      661 ttaaaaaagg gaagtggcag tggtgaagag ctactatcca agaattatca tcttgagaac
      721 gaggtagcgc gtttgaagaa gggttccggc tcaggagagg aactgctctc gaagaactat
      781 catcttgaaa atgaggtcgc tcgattaaaa aagggatcgg gcagtggtga ggaactactt
      841 tcaaagaatt accacctcga aaacgaagta gctcgattaa agaaaggttc agggtcgggt
      901 gaagaattac tga
//
