LOCUS       sequence-99838-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fusion protein of the SunTag10xGCN4 v4 peptide array to a 
            mitochondrial targeting domain and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-mito-mCherry-10xGCN4_v4
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             156..212
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             228..284
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             300..356
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             372..428
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             444..500
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             516..572
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             588..644
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             660..716
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             732..788
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             804..860
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
       61 gcggcatgga cgagctgtac aagggtggtg gtggcgcggc cgcagatcga tcgatcacgc
      121 gtgccaccat gactagtttt gtacaaagcg gtccggaaga acttttgagc aagaattatc
      181 atcttgagaa cgaagtggct cgtcttaaga aaggttctgg cagtggagaa gaactgcttt
      241 caaagaatta ccacctggaa aatgaggtag ctagactgaa aaaggggagc ggaagtgggg
      301 aggagttgct gagcaaaaat tatcatttgg agaacgaagt agcacgacta aagaaagggt
      361 ccggatcggg tgaggagtta ctctcgaaaa attatcatct cgaaaacgaa gtggctcggc
      421 taaaaaaggg cagtggttct ggagaagagc tattatctaa aaactaccac ctcgaaaatg
      481 aggtggcacg cttaaaaaag ggaagtggca gtggtgaaga gctactatcc aagaattatc
      541 atcttgagaa cgaggtagcg cgtttgaaga agggttccgg ctcaggagag gaactgctct
      601 cgaagaacta tcatcttgaa aatgaggtcg ctcgattaaa aaagggatcg ggcagtggtg
      661 aggaactact ttcaaagaat taccacctcg aaaacgaagt agctcgatta aagaaaggtt
      721 cagggtcggg tgaagaatta ctgagtaaaa attatcatct ggaaaatgag gtagcgagac
      781 taaaaaaggg gagtggttct ggcgaggaat tgctatcgaa aaattatcat cttgagaacg
      841 aagttgctag gctcaaaaag ggctcaggct caggcaccgc ggtaaacata tggatcgatc
      901 ataatactcg agtctaga
//
