LOCUS       sequence-99843-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A minimal K560 kinesin rigor motor tagged with the SunTag for single
            molecule imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..21
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     52..76
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
       61 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      121 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttggta ccgagctcgg
      181 atccgccacc atggcggacc tggccgagtg caacatcaaa gtgatgtgtc gcttcagacc
      241 tctcaacgag tctgaagtga accgcggcga caagtacatc gccaagtttc agggagaaga
      301 cacggtcgtg atcgcgtcca agccttatgc atttgatcgg gtgttccagt caagcacatc
      361 tcaagagcaa gtgtataatg actgtgcaaa gaagattgtt aaagatgtac ttgaaggata
      421 taatggaaca atatttgcat acggccaaac atcctctggg aagacacaca caatggaggg
      481 taaacttcat gatccagaag gcatgggaat tattccaaga atagtgcaag atatttttaa
      541 ttatatttac tcgatggatg aaaatttgga atttcatatt aaggtttcat attttgaaat
      601 atatttggat aagataaggg acctgttaga tgtttcaaag accaaccttt cagttcatga
      661 agacaaaaac cgagttccct atgtaaaggg gtgcacagag cgttttgtat gtagtccaga
      721 tgaagttatg gataccatag atgaaggaaa atccaacaga catgtagcag ttacaaatat
      781 gaatgaacat agctctagga gtcacagtat atttcttatt aatgtcaaac aagagaacac
      841 acaaacggaa caaaagctga gtggaaaact ttatctggtt gatttagctg gtagtgcgaa
      901 ggttagt
//
