LOCUS       sequence-99844-e         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from
            the U6 promoter and a puromycin resistance cassette and BFP from the
            EF1Alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pU6-sgRNA EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(300..333)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     337..357
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
       61 gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc gacgcgccat
      121 ctctaggccc gcgccggccc cctcgcacgg acttgtggga gaagctcggc tactcccctg
      181 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa
      241 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta
      301 taacttcgta tagcatacat tatacgaagt tataaacagc acaaaaggaa actcacccta
      361 actgtaaagt aattgtgtgt tttgagacta taagtatccc ttggagaacc accttgttgg
      421 accaggatgg gcaccacccg tttaagagct aagctggaaa cagcatagca agtttaaata
      481 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctcgagta
      541 cta
//
