LOCUS       sequence-99846-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A minimal K560 kinesin rigor motor tagged with the SunTag for single
            molecule imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..128
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             144..209
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             225..290
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             306..371
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             387..452
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             468..533
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             549..614
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             630..695
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             711..776
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             792..857
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 atcttgagaa cgaagtggct cgtcttaaga aactcgtagg agaaagaggt ggcagtggag
       61 gtctgcttcc caagaattac cacctggaaa atgaggtagc tagactgaaa aagctcgtag
      121 gcgagcgcgg gggaagtggg ggattgctgc caaaaaatta tcatttggag aacgaagtag
      181 cacgactaaa gaaattagtt ggggagaggg ggggatcggg tggcttactc ccgaaaaatt
      241 atcatctcga aaacgaagtg gctcggctaa aaaagttagt tggagagcgt ggcggttctg
      301 gaggcctatt accaaaaaac taccacctcg aaaatgaggt ggcacgctta aaaaagctgg
      361 tcggagaacg gggaggcagt ggtggcctac tacccaagaa ttatcatctt gagaacgagg
      421 tagcgcgttt gaagaagctc gtcggtgaaa gaggtggctc aggagggctg ctcccaaaga
      481 actatcatct tgaaaatgag gtcgctcgat taaaaaagtt agtgggcgag aggggaggca
      541 gtggtgggct acttcctaag aattaccacc tcgaaaacga agtagctcga ttaaagaaat
      601 tggtcggtga gagaggtggg tcgggtggct tactgccgaa aaattatcat ctggaaaatg
      661 aggtagcgag actaaaaaag ctcgtaggtg agagaggggg ttctggcggc ttgctaccaa
      721 aaaattatca tcttgagaac gaagttgcta ggctcaaaaa gctggttgga gagcgcggcg
      781 gctcaggcgg tctgttaccc aaaaactacc acttggaaaa tgaggttgcc agattgaaga
      841 aactggtcgg cgagcgcggt ggaagtggag gactacttcc taaaaattat catttggaga
      901 acgaagtcgc tagactg
//
