LOCUS       sequence-99847-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A minimal K560 kinesin rigor motor tagged with the SunTag for single
            molecule imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             293..358
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             374..439
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             455..520
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             536..601
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             617..682
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             698..763
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             779..844
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 agttttacag gccctagaag aacttgctgt caattatgat cagaagtctc aggaagttga
       61 agacaaaact aaggaatatg aattgcttag tgatgaattg aatcaggaat cggcaacttt
      121 agcgagtata gatgctgagc ttcagaaact taaggaaatg accaaccacc agaaaaaacg
      181 agcagctgag atgatggcat ctttactaaa aggtaccgcg gccgcagatc gatcgatcac
      241 gcgtgccacc atgactagtt ttgtacaaag cggtccggga ggttctggag gattgttgcc
      301 caaaaactac cacttggaga acgaggtcgc aaggttgaaa aaattggtcg gtgagcgtgg
      361 cggctcaggc ggccttcttc caaaaaatta tcatcttgaa aatgaagttg ctagacttaa
      421 aaaacttgtt ggagaaagag gcgggagcgg cggcctgctg cccaagaact accacctgga
      481 gaacgaggtc gcccgcctga agaagctggt cggcgagcgc ggtggttctg gaggtttgct
      541 cccaaagaac taccacttgg aaaacgaagt cgcaagattg aagaagttgg tcggtgaaag
      601 aggaggaagc ggaggattat tacctaaaaa ttatcatttg gaaaatgaag ttgctagatt
      661 gaagaagttg gttggagaga gaggggggtc tggaggacta ctaccgaaaa actaccacct
      721 agagaacgag gtggcccgac taaaaaagct agtgggcgag cgcggcggca gtggcggcct
      781 cctccccaag aattaccacc tcgaaaatga agtagcaagg ctcaagaagc tcgtaggaga
      841 aagaggtggt tccgggggac tccttccaaa aaactatcat cttgaaaacg aagttgcacg
      901 tctt
//
