LOCUS       sequence-99848-e         385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9
            fusion protein and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pHR-SFFV-KRAB-dCas9-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa
      121 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
      181 ttggcaagct agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa
      241 tagagaagtt cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat
      301 atctgcggtg agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt
      361 cggccccggc ccgaggccaa gaaca
//
