LOCUS       sequence-99850-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A minimal K560 kinesin rigor motor tagged with the SunTag for single
            molecule imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..76
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             92..157
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             173..238
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             254..319
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             335..400
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             416..481
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             497..562
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             578..643
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 ttctggaggc ctattaccaa aaaactacca cctcgaaaat gaggtggcac gcttaaaaaa
       61 gctggtcgga gaacggggag gcagtggtgg cctactaccc aagaattatc atcttgagaa
      121 cgaggtagcg cgtttgaaga agctcgtcgg tgaaagaggt ggctcaggag ggctgctccc
      181 aaagaactat catcttgaaa atgaggtcgc tcgattaaaa aagttagtgg gcgagagggg
      241 aggcagtggt gggctacttc ctaagaatta ccacctcgaa aacgaagtag ctcgattaaa
      301 gaaattggtc ggtgagagag gtgggtcggg tggcttactg ccgaaaaatt atcatctgga
      361 aaatgaggta gcgagactaa aaaagctcgt aggtgagaga gggggttctg gcggcttgct
      421 accaaaaaat tatcatcttg agaacgaagt tgctaggctc aaaaagctgg ttggagagcg
      481 cggcggctca ggcggtctgt tacccaaaaa ctaccacttg gaaaatgagg ttgccagatt
      541 gaagaaactg gtcggcgagc gcggtggaag tggaggacta cttcctaaaa attatcattt
      601 ggagaacgaa gtcgctagac tgaaaaagtt ggttggggaa cgaggagggt ctggcggcaa
      661 gctagtcgaa ttcgacacgt aggctagctt agtaggcggc cgcccccccc ctaacgttac
      721 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
      781 attgccgtct tttggcaatg tgagggcccg gaaacct
//
