LOCUS       sequence-99853-e         413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9
            fusion protein and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pHR-SFFV-KRAB-dCas9-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttctccgctg tcgaacagca gggcgccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcca
      301 tcgaccctcc gccggaaccg ccgggctctt ctcccttctc caaccggagg atcacatctg
      361 gcttagtaag ctgataaccc aaggaaacca ggttcttata gttctccagc atc
//
