LOCUS       sequence-99858-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A fusion protein of the SunTag 24xGCN4 v1 peptide array to a 
            mitochondrial targeting domain and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-mito-mCherry-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(531..549)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     799..818
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc
      181 caccatgagt ctgacttcca gttccagcgt acgagttgaa tggatcgcag cagttaccat
      241 tgctgctggg acagctgcaa ttggttatct agcttacaaa agattttatg ttaaagatca
      301 tcgaaataaa gctatgataa accttcacat ccagaaagac aaccccaaga tagtacatgc
      361 tggaagctta ccggtcgccg aattcggtgt gagcaagggc gaggaggata acatggccat
      421 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      481 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      541 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      601 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      661 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      721 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      781 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggct
//
