LOCUS       sequence-99869-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Violin JD, Zhang J, Tsien RY, Newton AC
  TITLE     A genetically encoded fluorescent reporter reveals oscillatory 
            phosphorylation by protein kinase C.
  JOURNAL   J Cell Biol. 2003 Jun 9. 161(5):899-909.
  PUBMED    12782683
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jun 9. 161(5):899-909."
COMMENT     Sequence Label: CKAR
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..128
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(729..980)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     primer_bind     complement(771..792)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
       61 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      121 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac tccaccagat
      181 ccacctgcct tagccttgat cttcaacgtc tggaatcttc tgaatcgacc tccggatcct
      241 cctaactttt tcaccaaatc tttttcttcg gctgtttgct taagtactac tctttgttct
      301 tgtaacatac ctaatccctc gttaaacaga cctgtagtat cgttaatttc cactttaaag
      361 ccaatgacaa atttattgtt tttatcccaa atgatcttga tttcatctcc gtcttgtaaa
      421 aggaatttcg taccctgtat catgcggtta ttatttaaat agctcacgtt agttccggtg
      481 tggcaatacc aaatatcatc taaaccttgt gccggagatt catacatgct tttgcctaca
      541 gcatgcctct ttttgaaaat gaagcaatga actcgagaca acctactgtc ttcaatttta
      601 caattgcaat cctcggatct accaatgaaa aatggattca caccttgctg aatctccagg
      661 ctttcttgaa taatgctgtc aggcaatggt tttaaagtta aaaacctacc attacccttg
      721 gtaccagcct tgtacagctc gtccatgccg tgagtgatcc cggcggcggt cacgaactcc
      781 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc
      841 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      901 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gtgggccttg
      961 atgccgttct tctgcttgtc ggc
//
