LOCUS       sequence-99872-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Violin JD, Zhang J, Tsien RY, Newton AC
  TITLE     A genetically encoded fluorescent reporter reveals oscillatory 
            phosphorylation by protein kinase C.
  JOURNAL   J Cell Biol. 2003 Jun 9. 161(5):899-909.
  PUBMED    12782683
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jun 9. 161(5):899-909."
COMMENT     Sequence Label: CKAR
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(34..747,748..750))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     428..447
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttaggtgac actatagaat agggccctct agattacttg tacagctcgt ccatgccgtg
       61 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      121 gtctttgctc agcttggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc
      181 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      241 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
      301 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      361 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      421 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      481 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      541 gcacatcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      601 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      661 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      721 cccggtgaac agctcctcgc ccttgctcac tccaccagat ccacctgcct tagccttgat
      781 cttcaacgtc tggaatcttc tgaatcgacc tccggatcct cctaactttt tcaccaaatc
      841 tttttcttcg gctgtttgct taagtactac tctttgttct tgtaacatac ctaatccctc
      901 gttaaacaga cctgtagtat cgttaatttc cactttaaag ccaatgacaa atttattgtt
      961 tttatcccaa atgatcttga tttcatctcc gtcttgtaaa aggaa
//
