LOCUS       sequence-99873-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Violin JD, Zhang J, Tsien RY, Newton AC
  TITLE     A genetically encoded fluorescent reporter reveals oscillatory 
            phosphorylation by protein kinase C.
  JOURNAL   J Cell Biol. 2003 Jun 9. 161(5):899-909.
  PUBMED    12782683
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jun 9. 161(5):899-909."
COMMENT     Sequence Label: CKAR
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(95..97,98..811)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(137..158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(398..417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     745..766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(827..845)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(828..845)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(865..882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    871..982
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaagatttg gtgaaaaagt taggaggatc cggaggtcga ttcagaagat tccagacgtt
       61 gaagatcaag gctaaggcag gtggatctgg tggagtgagc aagggcgagg agctgttcac
      121 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      181 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      241 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
      301 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      361 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      421 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      481 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      541 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      601 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      661 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa
      721 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      781 cactcacggc atggacgagc tgtacaagta atctagaggg ccctattcta tagtgtcacc
      841 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggg
//
