LOCUS       sequence-99883-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""CoinFLP-Gal4"" - Expresses Gal4 from actin promoter downsteam of 
            transcriptional stop. Recombination by FLP excises FRT-FRT pair to 
            express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Bosch JA, Tran NH, Hariharan IK
  TITLE     CoinFLP: a system for efficient mosaic screening and for visualizing
            clonal boundaries in Drosophila.
  JOURNAL   Development. 2015 Feb 1;142(3):597-606. doi: 10.1242/dev.114603.
  PUBMED    25605786
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2015-02-1- 1"; volume: "142"; 
            issue: "3"; pages: "597-606"
COMMENT     Sequence Label: pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(227..260)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(301..334)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(355..388)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(764..797)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 agtagcgaca ctcccagttg ttcttcagac acttggcgca cttcggtttt tctttggagc
       61 acttgagctt tttaagtcgg caaatatcgc atgcttgttc gatagaagac agtagcttca
      121 tctttcagga ggcttgcttc aagcttgggc tgcagattgt ttagcttgtt cagctgcgct
      181 tgtttatttg cttaagatct gaattccacc gcaacactac accatcgaag ttcctatact
      241 atttgaagaa taggaacttc aagcgagccg tagtgatttg gatgtatggt ggtaagcggg
      301 gaagttccta tactttctag agaataggaa cttcccaaat acgctccctc acacgaagtt
      361 cctatactat ttgaagaata ggaacttccg gcagacaaag caataaggac tgatcacttt
      421 aagggccgtt acgtcacgta ctgccttgga agtagtcgtc aactcctcta tgcctgaacc
      481 cagcggccgc tggccggccg caactagaac tagtggatct aaacgagttt ttaagcaaac
      541 tcactccctg acaataaaaa cgcaattatc tttaaataaa tatattctat ttattaacca
      601 agtagctaat atagctaaaa tcaatttgtt gctaacttaa aactaacata attatgtatt
      661 agttatttac attcttttgg ttggttacaa ccaaataaat aatatcactt ttaaaaactt
      721 aagccaggaa ctgaataatt ttctatttgg ctttagtcaa ttcgaagttc ctatactttc
      781 tagagaatag gaacttccgg gatcctctag ctagagtccc ctcgagggga cctgcaggca
      841 tgcaagctga tccactagcc gtacccgggg atcgatcctg taagctgcaa tggaaagaat
      901 gcggatttcc cattagtttt tggttacc
//
