LOCUS       sequence-99886-e         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and 
            BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pLV-sgCDKN1B#2 BFP
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..95
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gcacctggtg catgacccgc aagcccggtg ccggatcggg agagggcaga ggaagtctgc
       61 taacatgcgg tgacgtcgag gagaatcctg gcccaccggt cgccaccagc gagctgatta
      121 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
      181 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
      241 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
      301 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
      361 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      421 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
      481 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
      541 cgctg
//
