LOCUS       sequence-99889-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 
            peptide v4 and BFP. This plasmid is part of the SunTag system for 
            gene activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..75
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             55..81
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             205..261
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             277..333
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             349..405
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             421..477
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             493..549
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             565..621
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacgccta tccctatgac
       61 gtgcccgatt atgccagcct gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat
      121 cctaagaaaa agcggaaagt ggacggcatt ggtagtggga gcaacggcag cagcggatcc
      181 aacggtccga ctgacgccgc ggaagaagaa cttttgagca agaattatca tcttgagaac
      241 gaagtggctc gtcttaagaa aggttctggc agtggagaag aactgctttc aaagaattac
      301 cacctggaaa atgaggtagc tagactgaaa aaggggagcg gaagtgggga ggagttgctg
      361 agcaaaaatt atcatttgga gaacgaagta gcacgactaa agaaagggtc cggatcgggt
      421 gaggagttac tctcgaaaaa ttatcatctc gaaaacgaag tggctcggct aaaaaagggc
      481 agtggttctg gagaagagct attatctaaa aactaccacc tcgaaaatga ggtggcacgc
      541 ttaaaaaagg gaagtggcag tggtgaagag ctactatcca agaattatca tcttgagaac
      601 gaggtagcgc gtttgaagaa gggttccggc tcaggagagg aactgctctc gaagaactat
      661 catcttgaaa
//
