LOCUS       sequence-99890-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 
            peptide v4 and BFP. This plasmid is part of the SunTag system for 
            gene activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..106
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             122..178
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             194..250
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             266..322
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             338..394
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             410..466
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             482..538
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             554..610
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             692..712
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             722..778
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccacctggaa aatgaggtag ctagactgaa aaaggggagc ggaagtgggg aggagttgct
       61 gagcaaaaat tatcatttgg agaacgaagt agcacgacta aagaaagggt ccggatcggg
      121 tgaggagtta ctctcgaaaa attatcatct cgaaaacgaa gtggctcggc taaaaaaggg
      181 cagtggttct ggagaagagc tattatctaa aaactaccac ctcgaaaatg aggtggcacg
      241 cttaaaaaag ggaagtggca gtggtgaaga gctactatcc aagaattatc atcttgagaa
      301 cgaggtagcg cgtttgaaga agggttccgg ctcaggagag gaactgctct cgaagaacta
      361 tcatcttgaa aatgaggtcg ctcgattaaa aaagggatcg ggcagtggtg aggaactact
      421 ttcaaagaat taccacctcg aaaacgaagt agctcgatta aagaaaggtt cagggtcggg
      481 tgaagaatta ctgagtaaaa attatcatct ggaaaatgag gtagcgagac taaaaaaggg
      541 gagtggttct ggcgaggaat tgctatcgaa aaattatcat cttgagaacg aagttgctag
      601 gctcaaaaag ggctcaggct caggcaccgc ggtaaacata ggtggtggaa ccggtccgat
      661 ggatctacag cggccgcaag gtggaggtgg acccaagaag aagcgcaagg tgggaagcgg
      721 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgc
      781 accaggaagt atgagcgag
//
