LOCUS       sequence-99892-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v1 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-sfGFP-24xGCN4_v1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..127
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             143..208
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             224..289
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             305..370
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             386..451
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             467..532
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             548..613
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             629..694
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             710..775
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             791..856
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 tcttgagaac gaagtggctc gtcttaagaa actcgtagga gaaagaggtg gcagtggagg
       61 tctgcttccc aagaattacc acctggaaaa tgaggtagct agactgaaaa agctcgtagg
      121 cgagcgcggg ggaagtgggg gattgctgcc aaaaaattat catttggaga acgaagtagc
      181 acgactaaag aaattagttg gggagagggg gggatcgggt ggcttactcc cgaaaaatta
      241 tcatctcgaa aacgaagtgg ctcggctaaa aaagttagtt ggagagcgtg gcggttctgg
      301 aggcctatta ccaaaaaact accacctcga aaatgaggtg gcacgcttaa aaaagctggt
      361 cggagaacgg ggaggcagtg gtggcctact acccaagaat tatcatcttg agaacgaggt
      421 agcgcgtttg aagaagctcg tcggtgaaag aggtggctca ggagggctgc tcccaaagaa
      481 ctatcatctt gaaaatgagg tcgctcgatt aaaaaagtta gtgggcgaga ggggaggcag
      541 tggtgggcta cttcctaaga attaccacct cgaaaacgaa gtagctcgat taaagaaatt
      601 ggtcggtgag agaggtgggt cgggtggctt actgccgaaa aattatcatc tggaaaatga
      661 ggtagcgaga ctaaaaaagc tcgtaggtga gagagggggt tctggcggct tgctaccaaa
      721 aaattatcat cttgagaacg aagttgctag gctcaaaaag ctggttggag agcgcggcgg
      781 ctcaggcggt ctgttaccca aaaactacca cttggaaaat gaggttgcca gattgaagaa
      841 actggtcggc gagcgcggtg gaagtggagg actacttcct
//
