LOCUS       sequence-99898-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sfGFP and the 24xGCN4_v1 peptide array are expressed from the same 
            transcript, separated by a P2A site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v1-P2A-sfGFP
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..129
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             145..210
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             226..291
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             307..372
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             388..453
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             469..534
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             550..615
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             631..696
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             712..777
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             793..858
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 catcttgaga acgaagtggc tcgtcttaag aaactcgtag gagaaagagg tggcagtgga
       61 ggtctgcttc ccaagaatta ccacctggaa aatgaggtag ctagactgaa aaagctcgta
      121 ggcgagcgcg ggggaagtgg gggattgctg ccaaaaaatt atcatttgga gaacgaagta
      181 gcacgactaa agaaattagt tggggagagg gggggatcgg gtggcttact cccgaaaaat
      241 tatcatctcg aaaacgaagt ggctcggcta aaaaagttag ttggagagcg tggcggttct
      301 ggaggcctat taccaaaaaa ctaccacctc gaaaatgagg tggcacgctt aaaaaagctg
      361 gtcggagaac ggggaggcag tggtggccta ctacccaaga attatcatct tgagaacgag
      421 gtagcgcgtt tgaagaagct cgtcggtgaa agaggtggct caggagggct gctcccaaag
      481 aactatcatc ttgaaaatga ggtcgctcga ttaaaaaagt tagtgggcga gaggggaggc
      541 agtggtgggc tacttcctaa gaattaccac ctcgaaaacg aagtagctcg attaaagaaa
      601 ttggtcggtg agagaggtgg gtcgggtggc ttactgccga aaaattatca tctggaaaat
      661 gaggtagcga gactaaaaaa gctcgtaggt gagagagggg gttctggcgg cttgctacca
      721 aaaaattatc atcttgagaa cgaagttgct aggctcaaaa agctggttgg agagcgcggc
      781 ggctcaggcg gtctgttacc caaaaactac cacttggaaa atgaggttgc cagattgaag
      841 aaactggtcg gcgagcgcgg tggaagtgga ggactacttc ct
//
