LOCUS       sequence-99899-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sfGFP and the 24xGCN4_v1 peptide array are expressed from the same 
            transcript, separated by a P2A site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v1-P2A-sfGFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..118
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             134..199
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             215..280
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             296..361
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             377..442
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             458..523
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             539..604
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             620..685
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             701..766
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             821..877
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgaagtagca cgactaaaga aattagttgg ggagaggggg ggatcgggtg gcttactccc
       61 gaaaaattat catctcgaaa acgaagtggc tcggctaaaa aagttagttg gagagcgtgg
      121 cggttctgga ggcctattac caaaaaacta ccacctcgaa aatgaggtgg cacgcttaaa
      181 aaagctggtc ggagaacggg gaggcagtgg tggcctacta cccaagaatt atcatcttga
      241 gaacgaggta gcgcgtttga agaagctcgt cggtgaaaga ggtggctcag gagggctgct
      301 cccaaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagttag tgggcgagag
      361 gggaggcagt ggtgggctac ttcctaagaa ttaccacctc gaaaacgaag tagctcgatt
      421 aaagaaattg gtcggtgaga gaggtgggtc gggtggctta ctgccgaaaa attatcatct
      481 ggaaaatgag gtagcgagac taaaaaagct cgtaggtgag agagggggtt ctggcggctt
      541 gctaccaaaa aattatcatc ttgagaacga agttgctagg ctcaaaaagc tggttggaga
      601 gcgcggcggc tcaggcggtc tgttacccaa aaactaccac ttggaaaatg aggttgccag
      661 attgaagaaa ctggtcggcg agcgcggtgg aagtggagga ctacttccta aaaattatca
      721 tttggagaac gaagtcgcta gactgaaaaa gttggttggg gaacgaggag ggtctggcgg
      781 caagctagcg atcggatcga tcgccgcgga aggaagcgga gctactaact tcagcctgct
      841 gaagcaggct ggagacgtgg aggagaaccc tggacctagc ggtagcaaag gag
//
