LOCUS       sequence-99900-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sfGFP and the 24xGCN4_v1 peptide array are expressed from the same 
            transcript, separated by a P2A site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v1-P2A-sfGFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     250..270
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga
       61 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc
      121 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      181 accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg
      241 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
      301 gctctacaaa ggttaaaccg gttgtacagg taccggatcc taaggtacct aattgcctag
      361 aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat
      421 atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta
      481 gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca
      541 tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct
      601 agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc
      661 atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc
      721 tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag
      781 catatgggct cgctggctgc agtattcccg ggttcattag atctcgcgaa gggcgaattc
      841 acgaggcgcg cccggatcgc gatcgcaatc ttaattaact cgagtctaga
//
