LOCUS       sequence-99901-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Tetracycline repressor expression vector, CMV 
            promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV TetR Blast (716-1)
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     633..652
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgagcg gccgccactg tgctggatat caaccacttt
       61 gtacaagaaa gctgggtcta gaattcccgg gatccgctgt acgcggaccc actttcacat
      121 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
      181 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
      241 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
      301 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
      361 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
      421 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
      481 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
      541 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
      601 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
      661 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atc
//
