LOCUS       sequence-99902-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 with single cysteine residue (M1C) for site-specific labelling
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, 
            Doudna JA
  TITLE     Programmable RNA recognition and cleavage by CRISPR/Cas9.
  JOURNAL   Nature. 2014 Sep 28. doi: 10.1038/nature13769.
  PUBMED    25274302
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Sep 28. doi: 10.1038/nature13769."
COMMENT     Sequence Label: 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S...
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atggttcttc tatgaaaatc gaagaaggta aactggtaat ctggattaac
      181 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
      241 attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca
      301 actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa
      361 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
      421 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg
      481 ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
      541 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
      601 gaaccgtact tcacctggcc gctgattgct gctgacgggg gt
//
