LOCUS       sequence-99904-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A direct fusion of sfGFP to the 24xGCN4_v4 peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-sfGFP
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..109
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             125..181
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             197..253
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             269..325
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             341..397
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             413..469
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             485..541
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             557..613
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             629..685
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             701..757
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             845..901
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg gcgaagaact
       61 cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg gcagtggcag
      121 cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga gattgaagaa
      181 agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga acgaagtcgc
      241 caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact accacttgga
      301 aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat tgagcaaaaa
      361 ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg gggaggagct
      421 cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag gctcggggag
      481 cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc gcttgaagaa
      541 agggagcggg agcggggaag agttactatc taagaattat catctcgaga acgaggtggc
      601 tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact atcatcttga
      661 aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac tcagtaagaa
      721 ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag gcgaagaact
      781 gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg gaagtggcag
      841 tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc gacttaaaaa
      901 gggttctggc tcgggtcagc ggccgcaccg gtaccccgcg gaaagcggta gcaaaggaga
      961 agaactt
//
