LOCUS       sequence-9991-ex        9698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Yu RC, Pesce CG, Colman-Lerner A, Lok L, Pincus D, Serra E, Holl M, 
            Benjamin K, Gordon A, Brent R
  TITLE     Negative feedback that improves information transmission in yeast 
            signalling.
  JOURNAL   Nature. 2008 Dec 11. 456(7223):755-61.
  PUBMED    19079053
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2008 Dec 11. 456(7223):755-61."
COMMENT     Sequence Label: pRY1001
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3286..3314)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3909..3929
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      6208..6395
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6263..6287)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6535..6551)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6535..6551)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6583..6602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6584..6602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6609..6626)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6609..6625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6618..6640)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6770..7225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6857..6876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7067..7088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7525..7929
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7942..9036
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9529..9548)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9648..9670
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggccgag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg
     2281 ttcgatagtc actccattgg acgtagaaaa ctctttatta cagaagttgt ccgcaaagag
     2341 tttgaagata tccctaacaa agcggaacaa gttccagttc cgccatcgag aaatcgtcaa
     2401 gagtatgcaa ggagtttatc acaaccatca caactccgtt aatcaagagc aggtcttgaa
     2461 cgttttgttc gagaatagca agcaagttct tttaggttta aaagatatgt tgaaaagcga
     2521 cgggcaaccc acttacttac atctgaaaat tgcaagttac attctttgta taacgaacgt
     2581 taaggaaccc ataaagctaa agacatttgt tgaaaacgaa tgtaaagaat tggtccagtt
     2641 tgcacaagac accctgaaga acttcgttca gtaatcactt tcaagctgta gtatgtaaac
     2701 gatatagatg aagttttcgt gtgtataaat atatgaactc tagagtgttg cataatttga
     2761 aacacagcat ttcttttcgg agagctcgtt tcaaaatgaa acaaacgccg ttgtccgttt
     2821 tcgtctcaat agaaaaagtg aaacagataa aaattgtttt aaaagaaacg aatttgcaac
     2881 atcttaagat atatcaaaac taatacaaaa cagcctaaaa aagattgaac aactcttcgc
     2941 ggtcaggtct cgacaccata aatcgaagta ctcgtacgct agttttctcg cacatagtac
     3001 cactacgttc cttttacaat tagattactt ctttttagtt gacttttttg agacgttcgt
     3061 gccattcata aaataggaaa agataacagg taagcactga agacttgttt tataagtgtc
     3121 ccaagcgaga cctagagtgg atattgatat ttctcaaaca agactcgtcg aagaaaacac
     3181 acttttatag cggaaccgct ttctttattt gaattgtctt gttcaccaag ggaattcgga
     3241 tccatggggc gcgccatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     3301 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     3361 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     3421 ccatggccaa cacttgtcac tactttaact tggggtgttc aatgcttttc aagataccca
     3481 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     3541 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     3601 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     3661 attcttggac acaaattgga atacaactat atttcacaca atgtatacat cactgcagac
     3721 aaacaaaaga atggaatcaa agctaacttc aaaactagac acaacattga agatggaagc
     3781 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     3841 ccagacaacc attacctgtc cacacaatct aaactttcga aagatcccaa cgaaaagaga
     3901 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggggatt
     3961 acacatggca tggatgaact atacaaggcg atcgctatga aagtccaaat aaccaatagt
     4021 agaacagagg aaatcttaaa agttcaagct aataatgaaa acgatgaagt cagtaaagct
     4081 actccgggcg aagttgaaga atcgctaagg ttaatcggcg atctaaaatt ctttttagcc
     4141 acagcgccgg taaattggca agaaaaccaa attataaggc gatactatct gaatagtgga
     4201 caaggctttg tctcttgtgt attttggaac aatctatact atattacagg tactgatatt
     4261 gtcaaatgtt gtctttacag aatgcaaaag tttgggagag aagtagttca aaagaaaaaa
     4321 tttgaagagg gtattttttc agatttaaga aatctcaaat gtggtataga tgcaacttta
     4381 gaacaaccaa agtccgaatt tttgtcgttt ctattcagaa atatgtgtct gaaaacccaa
     4441 aaaaagcaga aagtattttt ttggttcagt gtagcacatg ataagttgtt tgcggatgcg
     4501 ttggaaagag atttaaaaag agaaagtttg aatcagcctt caacgactaa gcccgttaat
     4561 gagcccgcct tatctttttc atatgattcc tcatctgata agcctctcta cgatcagtta
     4621 cttcaacatt tagattctag aagaccatct agtacaacaa aatcagataa ttcgcctcca
     4681 aaattagaaa gcgagaattt taaggataat gagttggtaa cagtaactaa tcagccgctt
     4741 ttaggcgttg gcctcatgga tgacgatgcg ccagaatccc cctctcaaat taatgatttt
     4801 attcctcaga aattgattat agaacccaat actctcgaat tgaatggtct cacagaagaa
     4861 acgcctcatg acttacccaa gaataccgct aagggcagag acgaagaaga ttttcctctc
     4921 gactattttc ctgtatctgt tgaataccct acggaggaaa atgcgtttga tccgttccct
     4981 ccacaggctt ttacgccagc tgccccttcc atgcctattt cctatgataa cgtgaatgaa
     5041 agggattcta tgcccgttaa ttctcttctt aatagatacc cctatcagtt atcagtggca
     5101 cccactttcc cagtgccacc atcatcatcg aggcaacatt ttatgacaaa tcgggatttt
     5161 tattcatcta acaataacaa ggaaaaattg gtatctccta gcgaccctac cagctacatg
     5221 aagtatgacg aaccagttat ggattttgat gaatctcggc caaatgaaaa ctgtacaaat
     5281 gcaaaatctc acaactctgg ccagcaaact aaacaacacc aattatattc taacaacttc
     5341 cagcaatctt acccaaacgg aatggttcca ggatactacc caaaaatgcc gtataatccc
     5401 atgggggggg atcctctact cgatcaagcc ttttatggcg cggacgattt tttctttcca
     5461 ccagaaggat gtgataacaa tatgctgtat ccacaaactg caacttcatg gaatgttttg
     5521 ccccctcaag ctatgcaacc agctccaacc tatgttggga gaccatacac accgaattat
     5581 agatcgacac caggttccgc gatgttccca tacatgcaaa gttcaaattc catgcagtgg
     5641 aacactgctg tttcacctta tagttcgaga gcaccatcta caactgctaa aaactatcct
     5701 cctagcacat tttattctca aaatataaat caatacccac ggcgaagaac tgtgggaatg
     5761 aagtcttcac aaggaaatgt tccaacaggt aataaacaat ctgtgggcaa gtctgcaaaa
     5821 atttcaaagc ctctacatat taagacaagt gcttatcaga agcaatacaa aatcaacttg
     5881 gaaacgaaag ccaggccaag tgctggtgac gaagattctg ctcatcctga taagaacaaa
     5941 gaaatttcga tgcctactcc ggattccaat actttggtgg tccagtcaga agaaggtgga
     6001 gctcattcac ttgaggtaga taccaatcga aggtccgata aaaaccttcc agatgcaacc
     6061 tgaactcgag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt
     6121 gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt
     6181 agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt
     6241 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     6301 cgaaaattct tgttcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     6361 catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc
     6421 gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg
     6481 tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcca
     6541 ctagttctag agcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat
     6601 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6661 cttaatcgcc ttgcagcaca tccccccttc gccagctggc gtaatagcga agaggcccgc
     6721 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt
     6781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7201 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     7261 gtatttcaca ccgcatatcg acggtcgagg agaacttcta gtatatccac atacctaata
     7321 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa
     7381 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa
     7441 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat
     7501 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga
     7561 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
     7621 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
     7681 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag aggccggaac
     7741 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga
     7801 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt
     7861 actttctaac ttttcttacc ttttacattt cagcaatata tatatatatt tcaaggatat
     7921 accattctaa tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac
     7981 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt
     8041 cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct
     8101 acaggtgtcc cacttccaga tgaggcgctg gaagcctcca agaaggttga tgccgttttg
     8161 ttaggtgctg tggctggtcc taaatggggt accggtagtg ttagacctga acaaggttta
     8221 ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc
     8281 gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt
     8341 gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat
     8401 ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg
     8461 gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct
     8521 aatcttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa
     8581 ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag
     8641 aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc
     8701 gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct
     8761 ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat
     8821 ttgccaaaga ataaggttga ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa
     8881 ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg
     8941 gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat
     9001 gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat
     9061 ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga
     9121 atatgttcat agggtagacg aaactatata cgcaatctac atacatttat caagaaggag
     9181 aaaaaggagg atagtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat
     9241 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa
     9301 gttaggtgta acagaaaatc atgaaactac gattcctaat ttgatattgg aggattttct
     9361 ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt
     9421 ttaagtcaat accttcttga agcatttccc ataatggtga aagttccctc aagaatttta
     9481 ctctgtcaga aacggcctta cgacgtagtc gatatggtgc actctcagta caatctgctc
     9541 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     9601 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     9661 gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
