LOCUS       sequence-99910-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The function of this material is to facilitate the capture and 
            expression of bacterial gene clusters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Yamanaka K, Reynolds KA, Kersten RD, Ryan KS, Gonzalez DJ, Nizet V, 
            Dorrestein PC, Moore BS
  TITLE     Direct cloning and refactoring of a silent lipopeptide biosynthetic 
            gene cluster yields the antibiotic taromycin A.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 
            10.1073/pnas.1319584111. Epub 2014 Jan 21.
  PUBMED    24449899
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1319584111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-4- 4"; volume: "111"; issue: "5"; pages: "1957-62"
COMMENT     Sequence Label: pCAP01
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..35)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    76..579
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
       61 atggtttctt agacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat
      121 ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta
      181 gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt
      241 taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat
      301 taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg
      361 tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc
      421 aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca
      481 aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat atatttatat
      541 taaaaaattt aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact
      601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ggtacctcaa
      661 gtctcgaggt tactagtcga tctttgtaga aaccatcggc gcagctattt acccgcagga
      721 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
      781 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtagatc
      841 aggcttcccg ggtgtctcgc tac
//
