LOCUS       sequence-99917-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid
            for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells
            and nuclei. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur
            M, Zhang F
  TITLE     In vivo interrogation of gene function in the mammalian brain using 
            CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
  PUBMED    25326897
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055."
COMMENT     Sequence Label: PX552
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     314..335
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
       61 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      121 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      181 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      241 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      301 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      361 tggacgagct gtacaagtcc ggactcagat ctcgagagga ggaggaggag acagacagca
      421 ggatgcccca cctcgacagc cccggcagct cccagccgag acgctccttc ctctcaaggg
      481 tgatcagggc agcgctaccg ttgcagctgc ttctgctgct gctgctgctc ctggcctgcc
      541 tgctacctgc ctctgaagat gactacagct gcacccaggc caacaacttt gcccgatcct
      601 tctaccccat gctgcggtac accaacgggc cacctcccac ctaggaattc gatatcaagc
      661 ttatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
      721 gtgcctctcc tggccctgga agttgccact ccag
//
