LOCUS       sequence-99918-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid
            for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells
            and nuclei. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur
            M, Zhang F
  TITLE     In vivo interrogation of gene function in the mammalian brain using 
            CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
  PUBMED    25326897
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055."
COMMENT     Sequence Label: PX552
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(402..423)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     751..770
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggccaggag aggcactggg gaggggtcac agggatgcca cccgtagatc tctcgagcag
       61 cgctcggtat cgataagctt gatatcgaat tcctaggtgg gaggtggccc gttggtgtac
      121 cgcagcatgg ggtagaagga tcgggcaaag ttgttggcct gggtgcagct gtagtcatct
      181 tcagaggcag gtagcaggca ggccaggagc agcagcagca gcagaagcag ctgcaacggt
      241 agcgctgccc tgatcaccct tgagaggaag gagcgtctcg gctgggagct gccggggctg
      301 tcgaggtggg gcatcctgct gtctgtctcc tcctcctcct ctcgagatct gagtccggac
      361 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      421 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      481 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      541 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      601 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      661 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      721 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      781 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      841 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtgg
//
