LOCUS       sequence-99921-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. The 
            N-terminal fragment is driven by PLac while the C-terminal fragment 
            is driven by PBAD. C-terminal fragment has the point mutations 
            R756S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSlb-R756S
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(82..109)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gcgtaatttc aacacgagcc aaaataataa aaaagcagga ttaataatct ggctttttat
       61 attctctctc tagtatataa acgcagaaag gcccacccga aggtgagcca gtgtgatcca
      121 agccagctcg atatccgtcg gcttgaacga attgttagac attacttgta gacgtgccct
      181 acgcgcttgt tgagttgttc ctcaacgttt ttcttgaagt gcttagcttc aaggtcacgg
      241 atacgaccga agcgagcctc gtcctcaatg gcccgaccga ttgcgcttgc tacagcctga
      301 acggttgtat tgtcagcact ggttaggcaa gccagagtgg tcttaatggt gatgtacgct
      361 acggcttccg gcttgatttc ttgcaggaac tggaaggctg tcgggcgctt gccgcgctta
      421 gctttcactt cctcaaacca gtcgttgatg cgtgcaatca tcttagggag tagggtagtg
      481 atgagaggct tggcggcagc gttatccgca acctcaccag ctttaagttg acgctcaaac
      541 atcttgcgga agcgtgcttc acccatctcg taagactcat gctcaagggc caactgttcg
      601 cgagctaaac gctcaccgta atggtcagcc agagtgttga acgggatagc agccagttcg
      661 atgtcagaga agtcgttctt agcgatgtta atcgtgttca tattagaaaa agaa
//
