LOCUS       sequence-99930-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enocdes a histone H2B fused to the SunTag for single molecule 
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pcDNA4TO-H2B-SunTag24x_v1
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     62..86
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             215..592
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             662..727
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             743..808
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             824..889
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             905..970
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
     CDS             986..1051
                     /codon_start=1
                     /product="GCN4 peptide that allows binding to an scFv-GFP 
                     fusion protein (Tanenbaum et al., 2014)"
                     /label=GCN4_v1
                     /translation="LLPKNYHLENEVARLKKLVGER"
ORIGIN
        1 taagcagagc tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac
       61 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      121 atagaagaca ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttggta
      181 ccgagctcgg atccggcgcg ccaccggtgc caccatgcca gagccagcga agtctgctcc
      241 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
      301 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
      361 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
      421 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
      481 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
      541 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatcgatc
      601 cggtggaggt ggaggtcctg caggttcgaa ggtacaaagc ggtccgggag gttctggagg
      661 attgttgccc aaaaactacc acttggagaa cgaggtcgca aggttgaaaa aattggtcgg
      721 tgagcgtggc ggctcaggcg gccttcttcc aaaaaattat catcttgaaa atgaagttgc
      781 tagacttaaa aaacttgttg gagaaagagg cgggagcggc ggcctgctgc ccaagaacta
      841 ccacctggag aacgaggtcg cccgcctgaa gaagctggtc ggcgagcgcg gtggttctgg
      901 aggtttgctc ccaaagaact accacttgga aaacgaagtc gcaagattga agaagttggt
      961 cggtgaaaga ggaggaagcg gaggattatt acctaaaaat tatcatttgg aaaatgaagt
     1021 tgctagattg aagaagttgg ttggagagag aggggggtct ggaggactac taccgaaaaa
     1081 ctac
//
