LOCUS       sequence-99934-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIalpha R 4-5
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             318..623
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     502..521
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    709..733
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
       61 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
      121 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
      181 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
      241 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
      301 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
      361 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
      421 ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc
      481 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg
      541 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
      601 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      661 gacggtctca ccgctgagtt gtcttctctg gtttaaacta tcagtgtttg acaggatata
      721 ttggcgggta aacctaagag aaaagagcg
//
