LOCUS       sequence-99946-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIalpha fin
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    15..39
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(110..415)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(212..231)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaaccag
       61 aaagtcttcg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
      121 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      181 cttccccgat aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      241 catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg
      301 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      361 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      421 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      481 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
      541 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
      601 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
      661 tcgggtatac atatcagtat atattcttat accgca
//
