LOCUS       sequence-99953-e       13233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible MyoD with mRNA tag for smFISH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Gibson TM, Gersbach CA
  TITLE     Single-molecule analysis of myocyte differentiation reveals bimodal 
            lineage commitment.
  JOURNAL   Integr Biol (Camb). 2015 Jun 8;7(6):663-71. doi: 10.1039/c5ib00057b.
            Epub 2015 May 8.
  PUBMED    25953198
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c5ib00057b"; journalName: "Integr Biol (Camb)"; date: 
            "2015-06-8- 8"; volume: "7"; issue: "6"; pages: "663-71"
COMMENT     Sequence Label: LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..13233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(92..112)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(510..521)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             714..947
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1019..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1056..1075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1110..1129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1159..1294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1326..1345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1327..1345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1355..1372)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1355..1371)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1516..1971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1603..1622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1813..1834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2000..2104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2105..2173,2174..2965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2323..2342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3136..3724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3625..3644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3878..3895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4012..4033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4048..4053,4054..4071,4072..4078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4086..4102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4091..4113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4110..4126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4110..4126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4145..4165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4147..4165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             4421..4601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4645..4770
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5263..5496
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5681..5725
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5992..6109
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(6142..6160,6161..6183,6184..6202,6203..6225,6226..6244,
                     6245..6267,6268..6286,6287..6309,6310..6328,6329..6351,
                     6352..6370,6371..6393,6394..6412)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        6445..6482
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6479..6503
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6621..6630
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7593..10637
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPISTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLPLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEGQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     promoter        10686..11196
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     11115..11133
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(11235..11852,11853..11858,11859..11894,11895..11897,
                     11898..11933,11934..11936,11937..11972,11973..11981)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(12026..12600,12601..12603,12604..12612)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12193..12210)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12420..12439
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12613..13212
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12613..12632)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13109..13129
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctagcttctg cagcacgtgt ctagaaacgc tggtcgacaa tcaacctctg gattacaaaa
       61 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      121 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      181 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      241 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      301 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
      361 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
      421 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
      481 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
      541 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
      601 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
      661 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      721 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      781 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      841 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      901 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
      961 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     1021 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1081 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1141 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     1201 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1261 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcgtcga gacgtaccca
     1321 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     1381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1501 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     1741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     1861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1921 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg
     1981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3241 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     4141 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     4201 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     4261 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     4321 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     4381 agagatattg tatttaagtg cctagctcga tacaataaac gggtctctct ggttagacca
     4441 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4501 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4561 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4621 ctgaaagcga aagggaaacc agagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     4681 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     4741 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     4801 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     4861 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     4921 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     4981 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     5041 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     5101 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     5161 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     5221 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     5281 gttcttggga gcagcaggaa gcactatggg cgcagcctca atgacgctga cggtacaggc
     5341 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     5401 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     5461 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     5521 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     5581 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     5641 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     5701 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     5761 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     5821 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     5881 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     5941 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     6001 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     6061 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
     6121 gactagcctc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     6181 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     6241 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     6301 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     6361 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     6421 agtcgagctc ggtacccggg tcgagtaggc gtgtacggtg ggaggcctat ataagcagag
     6481 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     6541 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt cgagctcggt acccggggat
     6601 cctctagtca gctgacgcgt gccaccatgg agcttctatc gccgccactc cgggacatag
     6661 acttgacagg ccccgacggc tctctctgct cctttgagac agcagacgac ttctatgatg
     6721 atccgtgttt cgactcacca gacctgcgct tttttgagga cctggacccg cgcctggtgc
     6781 acgtgggagc cctcctgaaa ccggaggagc acgcacactt ctctactgcg gtgcacccag
     6841 gcccaggcgc tcgtgaggat gagcatgtgc gcgcgcccag cgggcaccac caggcgggtc
     6901 gctgcttgct gtgggcctgc aaggcgtgca agcgcaagac caccaacgct gatcgccgca
     6961 aggccgccac catgcgcgag cgccgccgcc tgagcaaagt gaatgaggcc ttcgagacgc
     7021 tcaagcgctg cacgtccagc aacccgaacc agcggctacc caaggtggag atcctgcgca
     7081 acgccatccg ctacatcgaa ggtctgcagg ctctgctgcg cgaccaggac gccgcgcccc
     7141 ctggcgccgc tgccttctac gcacctggac cgctgccccc aggccgtggc agcgagcact
     7201 acagtggcga ctcagacgcg tccagcccgc gctccaactg ctctgatggc atgatggatt
     7261 acagcggccc cccaagcggc ccccggcggc agaatggcta cgacaccgcc tactacagtg
     7321 aggcggtgcg cgagtccagg ccagggaaga gtgcggctgt gtcgagcctc gactgcctgt
     7381 ccagcatagt ggagcgcatc tccacagaca gccccgctgc gcctgcgctg cttttggcag
     7441 atgcaccacc agagtcgcct ccgggtccgc cagagggggc atccctaagc gacacagaac
     7501 agggaaccca gaccccgtct cccgacgccg cccctcagtg tcctgcaggc tcaaacccca
     7561 atgcgattta tcaggtgctt atgcattgag ccgtcgtttt acaacgtcgt gactgggaaa
     7621 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7681 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7741 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
     7801 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
     7861 ccatctccac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
     7921 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
     7981 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
     8041 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
     8101 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
     8161 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
     8221 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg
     8281 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag
     8341 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
     8401 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
     8461 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
     8521 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg
     8581 aaaatggtct gctgccgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg
     8641 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
     8701 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
     8761 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc
     8821 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
     8881 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
     8941 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg
     9001 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
     9061 ttatttgccc gatgtacgcg cgcgtggatg aaggccagcc cttcccggct gtgccgaaat
     9121 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
     9181 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc
     9241 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
     9301 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
     9361 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga
     9421 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
     9481 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
     9541 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
     9601 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag
     9661 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cggccacatc agcgcctggc
     9721 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc
     9781 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
     9841 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
     9901 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag
     9961 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg
    10021 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgacgcggtg ctgattacga
    10081 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
    10141 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc
    10201 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
    10261 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
    10321 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
    10381 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
    10441 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
    10501 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggagacgact
    10561 cctggagccc gtcagtatcg gcggaattac agctgagcgc cggtcgctac cattaccagt
    10621 tggtctggtg tcaaaaagct agcgcggccg catcgataag cttgtggacg atatcgaatt
    10681 cgcacggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
    10741 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
    10801 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
    10861 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
    10921 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
    10981 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
    11041 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
    11101 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
    11161 tccctcgttg accgaatcac cgacctctct ccccaggggg gatccgttta tctgcagaat
    11221 tcgcccttga cgtcatgtct agactggaca agagcaaagt cataaacggc gctctggaat
    11281 tactcaatgg agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
    11341 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
    11401 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
    11461 aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg
    11521 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
    11581 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
    11641 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
    11701 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
    11761 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
    11821 gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg
    11881 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
    11941 acgctcttga cgattttgac cttgacatgc tccccgggta acggaccgga cgtcaagggc
    12001 gaattccagc acactgggaa ttccgcccct ctccctcccc cccccctaac gttactggcc
    12061 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
    12121 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
    12181 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
    12241 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
    12301 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
    12361 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
    12421 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
    12481 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
    12541 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
    12601 atggccacaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    12661 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    12721 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    12781 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    12841 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    12901 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    12961 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    13021 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    13081 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    13141 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    13201 cccggtgcct gaccagcaca ctggcggccg tta
//
