LOCUS       sequence-99954-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: 
            recommended as backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Myburgh R, Cherpin O, Schlaepfer E, Rehrauer H, Speck RF, Krause KH,
            Salmon P
  TITLE     Optimization of Critical Hairpin Features Allows miRNA-based Gene 
            Knockdown Upon Single-copy Transduction.
  JOURNAL   Mol Ther Nucleic Acids. 2014 Oct 28;3:e207. doi: 
            10.1038/mtna.2014.58.
  PUBMED    25350582
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2014-10-28"; volume: "3:e207. doi"; pages: "
            10.1038/mtna.2014.58"
COMMENT     Sequence Label: pENTRdeltaGFPmirGE-DroshaT1.5.5
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    175..274
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(325..339)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(438..452)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(551..565)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(696..717)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatgttgga atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc
       61 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
      121 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgcagctgg atggcaaata
      181 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
      241 cttataatgc caactttgta caagaaagct gggtctagag tagagtatgg tcaaccttac
      301 ttgattaaaa ctgtgaggtg ggaacatctg tggcttcact tcccacctcc cagttttaat
      361 aaggcactgc tgacattgct atcaccacta gagtagagta tggtcaacct tacttttccc
      421 tccacggcca gactttgcat ctgtggcttc accaaagtct ggtcgtggag ggagaggcac
      481 tgctgacatt gctatcacca ctagagtaga gtatggtcaa ccttactttt ccctccacgg
      541 ccagactttg catctgtggc ttcaccaaag tctggtcgtg gaggagaggc actgctgaca
      601 ttgctatcac cactagtatc gatggatcca cactggcgat tcaggccgct ttacttgtac
      661 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      721 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      781 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      841 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
      901 ttgtcggcc
//
