LOCUS       sequence-99955-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a membrane-targeted form of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Benediktsson AM, Schachtele SJ, Green SH, Dailey ME
  TITLE     Ballistic labeling and dynamic imaging of astrocytes in organotypic 
            hippocampal slice cultures.
  JOURNAL   J Neurosci Methods. 2005 Jan 30;141(1):41-53.
  PUBMED    15585287
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2005-01-30"; volume: "141(1)"; pages: "41-53"
COMMENT     Sequence Label: Lck-GFP
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(195..197,198..200,201..713)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(240..261)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(501..520)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctgccacca
       61 tgggctgtgg ctgcagctca aaccctgaag atgactggat ggagaacatt gacgtgtgtg
      121 agaactgcca ttatcccggc ggacgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      181 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      421 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgat
//
