LOCUS       sequence-99957-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta R E-F
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(157..162,163..180,181..187)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(307..326)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gattgtcttc aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
       61 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      121 gcggcccctc gaggggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
      181 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
      241 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      301 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      361 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      421 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
      481 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      541 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      601 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      661 aatatg
//
